Gibco

Mouse IL-28A (IFN-lambda 2) Recombinant Protein, PeproTech®

Mouse IL-28A (IFN-lambda 2) Protein in Functional Assay (FN)

FIGURE: 1 / 1

Mouse IL-28A (IFN-lambda 2) Protein (250-33-100UG) in FN

Bioassay analysis of Mouse IL-28A (IFN-lambda 2) Recombinant Protein, PeproTech® (Product # 250-33-1MG).

Product Image Gallery

Product Details

250-33-100UG

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

MDPVPRATRL PVEAKDCHIA QFKSLSPKEL QAFKKAKDAI EKRLLEKDMR CSSHLISRAW DLKQLQVQER PKALQAEVAL TLKVWENMTD SALATILGQP LHTLSHIHSQ LQTCTQLQAT AEPKPPSRRL SRWLHRLQEA QSKETPGCLE DSVTSNLFRL LTRDLKCVAS GDQCV

Molecular weight

19.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to activate STAT following receptor-ligand interaction.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine IFN- lambda2 is a 19.8 kDa protein containing 175 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes.

Cell

Karki R,Sharma BR,Tuladhar S,Williams EP,Zalduondo L,Samir P,Zheng M,Sundaram B,Banoth B,Malireddi RKS,Schreiner P,Neale G,Vogel P,Webby R,Jonsson CB,Kanneganti TD

250-33 was used in Enzyme-linked immunosorbent assay to show that only the combination of TNF-α and IFN-γ induced inflammatory cell death characterized by inflammatory cell death, PANoptosis while multiple inflammatory cytokines are produced by innate immune cells during SARS-CoV-2 infection.

Thu Jan 07 00:00:00 EST 2021

Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes.

Cell

Karki R,Sharma BR,Tuladhar S,Williams EP,Zalduondo L,Samir P,Zheng M,Sundaram B,Banoth B,Malireddi RKS,Schreiner P,Neale G,Vogel P,Webby R,Jonsson CB,Kanneganti TD

Thu Jan 07 00:00:00 EST 2021

PML-NB-dependent type I interferon memory results in a restricted form of HSV latency.

EMBO reports

Suzich JB,Cuddy SR,Baidas H,Dochnal S,Ke E,Schinlever AR,Babnis A,Boutell C,Cliffe AR

250-33 was used in In vitro experiments to demonstrate that viral genomes possess a memory of the IFN response during de novo infection, which results in differential subnuclear positioning and ultimately restricts the ability of genomes to reactivate.

Mon Sep 06 00:00:00 EDT 2021

IFN-λ Regulates Neutrophil Biology to Suppress Inflammation in Herpes Simplex Virus-1-Induced Corneal Immunopathology.

Journal of immunology (Baltimore, Md. : 1950)

Antony F,Pundkar C,Sandey M,Jaiswal AK,Mishra A,Kumar A,Channappanavar R,Suryawanshi A

Thu Apr 15 00:00:00 EDT 2021

IFN-λ Regulates Neutrophil Biology to Suppress Inflammation in Herpes Simplex Virus-1-Induced Corneal Immunopathology.

Journal of immunology (Baltimore, Md. : 1950)

Antony F,Pundkar C,Sandey M,Jaiswal AK,Mishra A,Kumar A,Channappanavar R,Suryawanshi A

Thu Apr 15 00:00:00 EDT 2021

Critical role of interferons in gastrointestinal injury repair.

Nature communications

McElrath C,Espinosa V,Lin JD,Peng J,Sridhar R,Dutta O,Tseng HC,Smirnov SV,Risman H,Sandoval MJ,Davra V,Chang YJ,Pollack BP,Birge RB,Galan M,Rivera A,Durbin JE,Kotenko SV

Tue May 11 00:00:00 EDT 2021

Spatiotemporal in vivo tracking of polyclonal human regulatory T cells (Tregs) reveals a role for innate immune cells in Treg transplant recruitment.

Molecular therapy. Methods & clinical development

Jacob J,Nadkarni S,Volpe A,Peng Q,Tung SL,Hannen RF,Mohseni YR,Scotta C,Marelli-Berg FM,Lechler RI,Smyth LA,Fruhwirth GO,Lombardi G

Fri Mar 12 00:00:00 EST 2021

Synergism of TNF-α and IFN-γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes.

bioRxiv : the preprint server for biology

Karki R,Sharma BR,Tuladhar S,Williams EP,Zalduondo L,Samir P,Zheng M,Sundaram B,Banoth B,Malireddi RKS,Schreiner P,Neale G,Vogel P,Webby R,Jonsson CB,Kanneganti TD

250-33 was used in In vitro experiments to reveal that blocking the COVID-19 cytokine-mediated inflammatory cell death signaling pathway identified in this study may benefit patients with COVID-19 or other cytokine storm-driven syndromes by limiting inflammation and tissue damage.

Fri Nov 13 00:00:00 EST 2020

Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells.

Scientific reports

Elias S,Robertson EJ,Bikoff EK,Mould AW

Wed Jan 10 00:00:00 EST 2018

SOCS1 is an inducible negative regulator of interferon λ (IFN-λ)-induced gene expression in vivo.

The Journal of biological chemistry

Blumer T,Coto-Llerena M,Duong FHT,Heim MH

Fri Oct 27 00:00:00 EDT 2017

Activation of type III interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta.

PloS one

Bierne H,Travier L,Mahlakõiv T,Tailleux L,Subtil A,Lebreton A,Paliwal A,Gicquel B,Staeheli P,Lecuit M,Cossart P

Thu Dec 13 00:00:00 EST 2012

IFN-lambda determines the intestinal epithelial antiviral host defense.

Proceedings of the National Academy of Sciences of the United States of America

Pott J,Mahlakõiv T,Mordstein M,Duerr CU,Michiels T,Stockinger S,Staeheli P,Hornef MW

Tue May 10 00:00:00 EDT 2011

The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Fernández de Marco Mdel M,Alejo A,Hudson P,Damon IK,Alcami A

Sat May 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: IFN-lambda-2; IFN-lambda2; IL-28A; ILN; Interferon lambda-2; interferon-lambda2; Interleukin; interleukin 28A; Interleukin-28A

Mouse IL-28A (IFN-lambda 2) Recombinant Protein, PeproTech®

Mouse IL-28A (IFN-lambda 2) Protein (250-33-100UG) in FN

Product Details

250-33-100UG

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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