Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Mouse IL-5 (ELISA Standard) Recombinant Protein
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
39-8051-65
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
Insect cells
Amino acid sequence
Mouse IL-5, amino acids Met21-Gly133 (Accession # NM_010558)
Molecular weight
32-34 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE
Conjugate
Form
Liquid
Purification
purified
Contains
0.09% sodium azide
Storage conditions
-80°C
Shipping conditions
Wet ice
Product Specific Information
Description: Recombinant mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Applications Reported: Recombinant mouse IL-5 is biologically active. The intended use of the Recombinant Mouse IL-5 Single-Use ELISA RSG Standard is as a standard for mouse IL-5 Ready-SET-Go! ELISA and mouse IL-5 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below.
Instructions for use: For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (500 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (1000 pg/mL).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 µL in 1.0 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-5 ELISA pair (TRFK5/TRFK4).
Source: Insect cells infected with baculovirus: amino acids Met 21-Gly 133 of mouse IL-5 accession # NM_010558.
Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: Il-5; Il5
UniProt ID: (Mouse) P04401
Entrez Gene ID: (Mouse) 16191
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support