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Invitrogen
Mouse IL-5 Recombinant Protein
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FIGURE: 1 / 1
Mouse IL-5 Protein (SMIL5) in ELISA Std
Sandwich ELISA analysis of human IL-5 was performed by coating wells of a 96-well plate with 110ul of a human IL-5 monoclonal antibody (Product # M551) in duplicate at 1, 2, 4, 5, 7, and 9ug/ml for at least 12 hours. The plate was blocked with a proprietary blocking solution for 1-4 hours at room temperature, and 50ul of mouse IL-5 recombinant protein (Product # SMIL5) was added in replicates of twelve at 400, 160, 64, 25.6, 10.24, and 0pg/ml for 1 hour at room temperature. The plate was washed and incubated 50ul of a biotinylated IL-5 m... View More
Product Details
SMIL5
Product Specifications
Expression System
Baculovirus
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Concentration
0.32 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
International reference standard is not available. Calibrated to a Thermo reference standard.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at 2-8°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
320 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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