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Invitrogen
Mouse Siglec-3 (CD33) Recombinant Protein
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Product Details
RP-88211
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse CD33, amino acids Met1-Glu240 (Accession # NP_067268.1) with six amino acids (LEVLFQ) at the C-terminus
Molecular weight
25.6 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Conjugate
Form
Lyophilized
Amount
200 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (800 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD33; FLJ00391; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3
Gene Aliases: Cd33; gp67; Siglec-3; Siglec3
UniProt ID: (Mouse) Q63994
Entrez Gene ID: (Mouse) 12489
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