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Invitrogen
Mouse Tumor Necrosis Factor Alpha Recombinant Protein
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FIGURE: 1 / 1
Mouse Tumor Necrosis Factor Alpha Protein (SMTNFA) in ELISA Std
Sandwich ELISA analysis of mouse TNF-alpha was performed using a Mouse TNF-alpha Colorimetric ELISA kit (Product # EMTNFA) by loading 50 ul per well of Mouse TNF-alpha Recombinant Protein (Product # SMTNFA) in dodecuplicate at 2450, 350, 50, and 0 pg/ml across a 3 ug/ml rat anti-mouse TNF-alpha (Product # MM350C ) pre-coated plate and incubating for 2 hours at room temperature along with 50 ul per well of r... View More
Product Details
SMTNFA
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Concentration
2.45 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This Mouse TNFalpha Standard is calibrated against NIBSC Mouse TNFalpha Reference Standard lot (8)8/532. 1 Thermo pg = 0.71 NIBSC pg = 0.14 NIBSC unit.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
2450 pg/ml when reconstituted at the recommend volume.
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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