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          • Proteins & Peptides ›
          • NeutrAvidin Proteins
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          NeutrAvidin Protein, DyLight™ 680

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          Cite NeutrAvidin Protein, DyLight™ 680

          Product Details

          22848

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000-1:25,000
          -

          Immunohistochemistry (IHC)

          1:200-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:200-1:1,000
          -

          ELISA (ELISA)

          1:100 - 1:500
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Type

          Protein

          Conjugate

          DyLight™ 680 DyLight™ 680 DyLight™ 680

          Excitation/Emission Max

          676/705 nm

          Form

          Liquid

          Concentration

          1 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Ambient

          Product Specific Information

          22848 contains 1 mL of protein in blue green colored liquid at a concentration of 1 mg/mL.

          Target Information

          Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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