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Gibco
Rat IL-10 Recombinant Protein
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Product Details
PRC0104
Product Specifications
Species
Rat
Expression System
E. coli
Amino acid sequence
Rat IL-10 recombinant protein contains 160 amino acids
Molecular weight
18.7 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 10 ng/mL; determined by the dose-dependent inhibition of antigen-specific T-cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
5mM tris, pH 7.2
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized rat IL-10 in 5 mM Tris, pH 7.2, to a concentration of 0.1-1.0 mg/mL. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
Gene Aliases: Il-10; Il10; IL10X
UniProt ID: (Rat) P29456
Entrez Gene ID: (Rat) 25325
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