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Invitrogen
SARS-CoV-2 Spike Protein (RBD) His-tag Recombinant Protein
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Product Details
RP-88397
Applications
Tested Dilution
Publications
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
SARS-CoV-2 spike RBD, amino acids Arg319-Phe541 (Accession # YP_009724390.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
26.54 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag) is 20-60 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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