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Invitrogen
SARS-CoV-2 Spike Protein (RBD) (aa330-524), His Tag Recombinant Protein
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Product Details
RP-87674
Applications
Tested Dilution
Publications
Product Specifications
Species
Virus
Expression System
E. coli
Amino acid sequence
SARS-CoV-2 Spike Protein (s-RBD) contains amino acids 330-524 of the SARS-CoV-2 Spike protein with a His-tag
Tag
His-tag
Molecular weight
27 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE under reducing conditions
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
Product Specific Information
RP-87674 has been tested in indirect ELISA.
Reconstitution: It is recommend that the vial be centrifuged briefly (~30 seconds) before opening the tube in order to bring the contents to the bottom. Then, reconstitute the protein with 400 µL of deionized sterile water to obtain a final concentration of 0.5 mg/mL. Let the tube sit on ice for ~30 minutes with gentle pipetting periodically.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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