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Invitrogen
TGF beta-2 Recombinant Protein
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Product Details
34-8478-82
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Human Transforming Growth Factor beta 2 (TGF beta 2) is a member of a superfamily of disulfide-linked homodimeric proteins secreted as latent proteins and stored at the cell surface and extracellular matrix. The bioactive TGF beta 2 is released from a latent complex by proteolytic processing and conformation changes. TGF beta 2 regulates cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix and is also involved in embryogenesis, tissue remodeling and wound healing.
Applications Reported: Human TGF beta 2 Recombinant Protein Carrier-Free is biologically active.
Applications Tested: The ED50 of this protein, as measured by inhibition of IL-4-dependent proliferation of mouse CTLL-2 cells is less than or equal to 1.5 ng/mL. This corresponds to a specific activity of greater than or equal to 6.7 x 10e5 Units/mg.
Source: E. coli expressed, accession number P61812, N-terminal His-tag.
Bioactivity: The ED50 of this protein, as measured by inhibition of IL-4-dependent proliferation of mouse CTLL-2 cells is less than or equal to 1.5 ng/mL. This corresponds to a specific activity of greater than or equal to 6.7 x 10e5 Units/mg.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: 25 kDa.
Storage and handling: For best results, quick-spin vial prior to opening. Use in a sterile environment.
Target Information
Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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