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Product Details
PEP-1456
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Class
Synthetic
Type
Peptide
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
purified
Storage buffer
PBS, pH 7.2, with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Dry ice
Product Specific Information
This peptide corresponds to the amino terminus of yeast Pst1.
PEP-1456 can be used as a blocking peptide with polyclonal antibody PA5-34414.
Target Information
Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain, which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell wall mannoprotein PST1; Haze protective factor 2; Protoplast secreted protein 1
Gene Aliases: D4214; HPF2; PST1; YD9609.09; YDR055W
UniProt ID: (Baker's yeast) Q12355
Entrez Gene ID: (Baker's yeast) 851625
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