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Krios 5 Cryo-TEM Features

Cryo-transmission electron microscope for high-fidelity atomic resolution cryo-EM

The 300 kV Thermo Scientific Krios 5 Cryo-Transmission Electron Microscope (Cryo-TEM) has been designed for high-performance single particle analysis (SPA), cellular cryo-electron tomography (cryo-ET) and microcrystal electron diffraction (MicroED). The Krios 5 Cryo-TEM is capable of quickly producing high-quality structures thanks to a unique focus on high throughput with high data fidelity, increased automation of routine alignments, improved optical precision, and AI-enabled software, which make it a highly productive and “ready-to-use” system for all expertise levels across multiple scientific fields.

Experience up to 25% faster throughput relative to previous Krios Cryo-TEM generations with high data fidelity for single particle analysis. Extended aberration-free image shift (AFIS) accelerates data collection, while AI-powered Thermo Scientific Smart EPU Software plugins automate routine alignments. Thermo Scientific CryoTomo Software Suite provides high image quality via automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms, unlocking the full potential of the Thermo Scientific Falcon 4i Direct Electron Detector beyond the Nyquist limit. Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Thermo Scientific Vacuum Capsule. Designed for sustainability, the Krios 5 Cryo-TEM features energy-saving Eco modes and environmental footprint traceability with a My Green Lab ACT Ecolabel.

Krios 5 Cryo-TEM benefits for cell and structural biology research

High-data-fidelity atomic-resolution cryo-EM

The Krios 5 Cryo-TEM offers up to 25% faster throughput with high data fidelity, extended aberration-free image shift (AFIS) and magnification calibration accuracy within 1% for single particle analysis. Combined with the optional Thermo Scientific Selectris X Imaging Filter and E-CFEG, the Krios 5 Cryo-TEM can deliver enhanced image contrast for rapid, atomic-resolution imaging.

Faster single particle analysis with AI-automation

AI-assistance with Smart EPU Software enables automated grid screening, quality monitoring, and data management. Smart Plugins in the Smart EPU Software use AI-based image analysis to automate grid area selection and dynamically adjust settings based on real-time analysis.

Accelerated, contamination-free cryo-electron tomography workflow

CryoTomo Software Suite provides automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms by unlocking the full data potential beyond the Nyquist limit.  Manage tomograms and experimental parameters with the Discovery Viewer in Thermo Scientific CryoFlow Software.  Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Vacuum Capsule.

Energy-saving Eco modes and My Green Lab ACT Ecolabel

The Krios 5 Cryo-TEM features energy-saving Eco modes with fast “wake up” scheduling, plus environmental impact certification with the My Green Lab® ACT® Ecolabel

Frequently asked questions about the Krios 5 Cryo-TEM

  • What is the Thermo Scientific Krios 5 Cryo-TEM? 
    The Krios 5 Cryo-TEM is a 300 kV cryogenic transmission electron microscope (cryo-TEM) engineered for high-fidelity cryo-electron microscopy (cryo-EM). It combines advanced optics, AI-enabled automation, and integrated software to support detailed 3D structural biology and cellular imaging workflows. 

  • What cryo-EM applications can the Krios 5 Cryo-TEM support? 
    The Krios 5 Cryo-TEM supports key structural biology methods, including single particle analysis, cryo-electron tomography (cryo-ET), and microcrystal electron diffraction (MicroED). These applications enable the high-resolution structural analysis of proteins and complex biological assemblies. 

  • How does the Krios 5 Cryo-TEM enhance data throughput and productivity? 
    The Krios 5 Cryo-TEM accelerates data acquisition through optimized optics, extended aberration-free imaging, and integrated automation. These enable up to ~25% faster throughput compared to previous Krios versions (use case dependent) without compromising image quality. 

  • What role does AI-enabled automation play in Krios 5 Cryo-TEM workflows? 
    AI-enabled automation simplifies routine cryo-EM tasks, including automated grid screening and dynamic feedback during data collection. This enhances consistency, reduces manual intervention, and improves overall workflow efficiency. 

  • What software is integrated with the Krios 5 Cryo-TEM? 
    The Krios 5 Cryo-TEM can leverage Thermo Scientific Smart EPU Software for automated single particle data acquisition, the Thermo Scientific CryoTomo Software Suite for tomographic data acquisition, and Thermo Scientific CryoFlow Software for comprehensive data management and visualization. These software solutions are specifically designed to streamline complex cryo-EM workflows. 

  • What hardware options can be used to enhance Krios 5 Cryo-TEM imaging performance? 
    The Krios 5 Cryo-TEM can leverage advanced direct electron detectors for high detective quantum efficiency in both single particle analysis and cryo-ET. Optional imaging filters and field emission sources can further improve contrast and resolution. 

  • How does the Krios 5 Cryo-TEM improve cryo-electron tomography workflows? 
    Leveraging the CryoTomo Software Suite, the Krios 5 Cryo-TEM enables on-the-fly motion correction and fast tilt series acquisition for subsequent high-resolution tomographic reconstruction. Combined with batch data handling in Tomo Multigrid Software, this accelerates the cryo-ET workflow and supports detailed 3D visualization of cellular structures. 

  • Can the Krios 5 Cryo-TEM support drug discovery research? 
    Yes. The Krios 5 Cryo-TEM can reveal atomic-level details on biological macromolecules and complexes, providing valuable insights on molecular interactions and mechanisms for drug discovery and therapeutics research. 

  • How does the Krios 5 Cryo-TEM support users of different skill levels? 
    The Krios 5 Cryo-TEM makes it easier for both novice and expert users to achieve reliable, high-quality cryo-EM data via automated, routine alignment, a user-friendly software interface, and a range of workflow templates. 

  • Where can researchers find support and learning resources for the Krios 5 Cryo-TEM?
    Thermo Fisher Scientific offers a rich library of product resources, including technical guides, webinars, and application notes that help you optimize workflows, troubleshoot common issues, and generally make the most out of your Krios 5 Cryo-TEM. 

Learn more about cryo-electron microscopy with the Krios 5 Cryo-TEM

Automation of daily alignments

Routine alignments, including beam and aperture centering as well as coma and astigmatism correction, are fully automated. Magnification calibration within 1% and a diagnostics dashboard ensure optimal maintenance.

Low-power Eco modes

The low-power Eco modes of the Krios 5 Cryo-TEM deactivate specific parts of the instrument when it is not in use, reducing power consumption up to 80%. The software can be programmed for early wake-up, ensuring the system is ready when needed.

Designed-in sustainability with the ACT Ecolabel

In addition to the energy-saving Eco modes of the Krios 5 Cryo-TEM, the My Green Lab ACT Ecolabel transparently documents the instrument’s environmental footprint, including manufacturing, energy and water use, as well as end-of-life disposal. At Thermo Fisher Scientific, our commitment to sustainability supports our mission to make the world healthier, cleaner, and safer.

Krios 5 Cryo-TEM imaging capabilities

Upgrade options for previous-generation Krios Cryo-TEMs

Electron source: cold field emission gun (E-CFEG)

 

E-CFEG is a low-energy spread cold field emission gun for higher contrast at truly atomic resolution. Low energy spread (≤0.3 eV) enables higher contrast at high resolutions (≤2.0 Å); Improved contrast at high spatial frequencies to reach higher resolutions in less time.

Comparison of a Thermo Scientific high-brightness field emission gun and the cold field emission gun on the Krios Cryo-TEM.

Krios Cryo-TEM accessories and software

 

Increase your lab’s productivity and performance with innovative upgrades:

Cryo-EM research in BSL-3 containment facilities

Work on contagious pathogens frequently requires specialized biosafety containment facilities. Until recently, cryo-EM equipment was not commonly found in these laboratories. At Thermo Fisher Scientific, we have developed products and services designed for these higher BSL facilities, so that our customers can accelerate their critical pathogen research with cryo-electron microscopy.

Cryo-EM in Biosafety Facilities for Virology Research

Krios Cryo-TEM technical specifications

Krios Cryo TEM Datasheet

Selectris Imaging Filters

Falcon 4i Direct Electron Detector

Vacuum Capsule datasheet

Smart EPU Software datasheet

CryoTomo Software Suite

For Research Use Only. Not for use in diagnostic procedures.