Seamless Cloning & Genetic Assembly
Tools for flexible protocols, and fast, high-quality results
- Speed—clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification
- Precision—no extra sequences; clone what you want where you want
- Flexibility—use our linearized vectors or any vector of your choice
- Simplicity—free online tool guides you through planning, design and assembly of your construct and PCR primer design, if needed
Expert Vector Construction: The GeneArt Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA. It’s what our experts use to make vectors, and they save 3-4 weeks versus traditional RE cloning methods.
Which Seamless Cloning & Assembly Kit is Right for You?
| Order now | Order now | Order now | Order now | Order now | |
| Assemble up to 4 fragments | Assemble up to 4 fragments | Assemble up to 4 fragments | Assemble up to 8 fragments | Assemble up to 10 fragments | |
|---|---|---|---|---|---|
| GeneArt Seamless Cloning Enzyme Mix | GeneArt Seamless Cloning & Assembly Kit | GeneArt Seamless PLUS Cloning and Assembly Kit | GeneArt Type IIs Assembly kits | GeneArt High-Order Genetic Assembly System | |
| Cloning Efficiency | >90% |
|
>90% |
|
>90% |
| All-in-one Enzyme/Buffer Mix | ✓ | ✓ | ✓ | ||
| # of Fragments Cloned Simultaneously | Up to 4 | Up to 4 | Up to 4 | Up to 8 | Up to 10 |
| Maximum Assembled Product Size | Up to 13 kb (with vector) | Up to 13 kb (with vector) | Up to 40 kb (with vector) |
|
Up to 110 kb (with vector) |
| Time to Clone Multiple Fragments | Typically in half a day | 1 day | Typically in half a day | Typically in half a day | 3 days |
| Replication Host | E. coli | E. coli | E. coli | E. coli | S. cerevisae MaV203 |
| Assemble Fragments w/o End Sequence Homology w/o PCR | ✓ | ✓ |
GeneArt Web-Based Design Tool
Guides the assembly of fragments, checks for potential issues, designs DNA oligonucleotides and presents an annotated sequence and graphical representation of the final assembled molecule.
Resources
Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications
For Research Use Only. Not for use in diagnostic procedures.