TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG, 250 reactions - FAQs

View additional product information for TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG - FAQs (4366073, 4366072, 4367846, 4364103, 4352042)

2 product FAQs found

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.