Applied Biosystems™

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Catalog number:  4352042

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. The optimized formulation contains AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX™ dye serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on Applied Biosystems® real-time PCR instruments.

Convenience: Everything you need for TaqMan®-based qPCR amplification and detection in a single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility: TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expression assays.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: AmpErase™, TaqMan™
Product Size: 250 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Wet Ice

Contents & storage

TaqMan® Fast Universal PCR Master Mix, No AmpErase® UNG Kit contains two tubes of 1.25 mL each sufficient for 250 20-µL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols