Mar 13, 2016
- Mar 16, 2016
Biology is complex and understanding it is a big challenge. Identify and quantify more proteins and complexities such as PTMs faster and more accurately with our new portfolio of LC-MS instruments, sample prep solutions, and software. High resolution/accurate mass (HR/AM) solutions using Thermo Scientific™ Orbitrap™ MS quantifies all detectable proteins and peptides with high specificity and fewer false positives, while triple quadrupole MS delivers SRM sensitivity and speed to detect targeted proteins more quickly. Join us in meeting today’s challenges. Together we’ll transform proteomics. Visit us at Booth #1.
Monday, March 14
Ultrasensitive Quantitative Profiling of Single FFPE Tumour Sections from Ovarian Cancers using TMT-MS3 on an Orbitrap Fusion MS for Clinical Research
Speaker: Gregg Morin, Ph.D.
Head, Proteomics, Michael Smith Genome Sciences Centre
Senior Scientist, British Columbia Cancer Agency
Associate Professor, Department of Medical Genetics, University of British Columbia
The rapid expansion of genome sequencing technology has driven the growth of research efforts focused on performing in-depth, quantitative genomic and proteomic analysis, on a per-individual basis. Although implementing this strategy would provide highly informative data for precision medicine research, the measurement of proteins in tumour and biopsy samples with mass spectrometry (MS)-based proteomics currently lags behind genome and transcriptome sequencing technologies. The limited adoption of proteomics for clinical research stems primarily from challenges related to sample processing (e.g., quantity of material required, type of material available (FFPE, frozen, OCT), throughput capabilities, and specialized technical expertise. Although recent research efforts as part of the CPTAC consortium or by independent groups have demonstrated large-scale analysis of cancer proteomes, these efforts have largely relied on the use of whole resected tumors from non-FFPE specimens. In a clinical research setting, it is not uncommon to obtain only a single needle-core biopsy, especially when dealing with rare or small tumours.
To overcome the challenges associated with proteomics profiling of small quantities of fixed tissue material, we have developed and optimized a complete analysis platform (termed SP3-CTP) that significantly simplifies sample processing and provides high-depth quantitative proteome analysis. SP3-CTP implements a solution-based approach to tissue disaggregation, de-crosslinking, lysis with SDS, digestion, and multiplexed quantification with tandem mass tag (TMT) labeling built on the ultrasensitive SP3 paramagnetic bead protocol, coupled to a Thermo Scientific Orbitrap Fusion™ Tribrid™ MS. To demonstrate the ultrasensitive capabilities of SP3-CTP, we will present the analysis of single 10 μm tumour tissue sections from a set of patients having high-grade serous, clear cell, or endometrioid ovarian cancer. These data demonstrate not only the quantitative depth that SP3-CTP coupled to TMT-MS3 analysis on an Orbitrap Fusion provides, but also the biological accuracy of the obtained quantitative information for research to differentiate ovarian cancer subtype expression dynamics.
For research use only. Not for use in diagnostic procedures.
All posters will be on display in Grand Ballroom B on Monday, March 14 and Tuesday, March 15.
- Automated, High-Throughput Hemoglobinopathies Profiling Using Top-Down LC-MS Methods
- Next-Generation Signaling Pathway Characterization by IS-PRM
- Pushing the Limits of Bottom-Up Proteomics with State-of-the-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes
- Quantitative Peptide Assay for Optimized and Reproducible Sample Preparations
- Quantitative Analysis of AKT/mTOR Pathway using Multiplex-Immunoprecipitation and Targeted Mass Spectrometry
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