Immunodiagnostic Assay Development with Dynabeads Plus

This 7 part diagram shares the value beyond the Dynabeads product from Security of supply to Analytics to Access to commercial rights

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Developing and validating an immunoassay can be complex due to the sensitivity, specificity, and precision required to enable highly reproducible assays. Time can often work against research groups developing these critical assays.

Learn more about how we can support your assay

How have Dynabeads Plus helped immunoassay development?

Dynabeads Plus team enhanced the sample preparation process for an Alzheimer’s disease diagnostic.

Customer need

How Dynabeads Plus helped

Results

Improve sample prep and cell enrichment for an Alzheimer’s disease diagnostic tool
Streamline and improve process for early diagnosis and monitoring tool for Alzheimer’s disease; specifically, increased sensitivity and validated markers

The Dynabeads team worked to achieve the required sensitivity goals, develop model assays, and initiate a supply and commercialization model. In addition, the team worked to structure collaboration through Work Packages.

Replaced the current ELISA assay, reduced the timeline for sample prep process, and contributed to improved cell enrichment in each sample
Co-developed an on-market assay targeting diagnostics, companion-diagnostics, and medical nutrition markets

Dynabeads immunodiagnostic product benefits include

High capacity & wide dynamic range
High signal-to-noise ratio
Adaptable to any known detection method & assay methodology
Efficient & reproducible ligand immobilization
Easy assay development
Direct or indirect capture approach
No magnetic remanence

Excellent kinetics & shorter assay times
Reproducible automated behavior
Fast and efficient washing
Increased sensitivity
Long reagent stability on the immunoassay analyzer
Easy handling in manufacturing and shortened assay development time

Assay Lifecycle Support

Feasibility

Evaluate your assay

Business support

Evaluation of multiple products at small-scale

Product support

Samples of different bead types
Samples coupled with antibody/antigen for rapid screening
Customized protocols

Development

Assist in your planning

Enterprise support

Evaluation of multiple products at small-scale
Collaboration and innovation with high quality products

Product support

Samples of different bead types
Samples coupled with antibody/antigen for rapid screening
Customized protocols
Bead material for robustness testing
Borderline bead material
Flexible supply volumes
Customized products
Short-term licensing and commercial supply quote agreements
Basic Quality Assurance Agreement (QAA)

Technical support

Technical support
Custom projects
Seminars
Assay automation support
Instrument supplier partnerships

Production

Grow your manufacturing

Enterprise support

Evaluation of multiple products at small-scale
Further investigations with selected product
Assay validation support
Joint development sessions to improve processes between our companies

Product support

Samples of different bead types
Samples coupled with antibody/antigen for rapid screening
Customized protocols
Borderline material
Flexible volumes
Customized products
Bead material from multiple batches for assay validation
Supply security through long-term commercial supply agreement (CSA)
Full Quality Assurance Agreement (QAA)

Technical support

Technical support
Custom projects
Seminars
Assay automation support
Instrument supplier partnerships

Post-launch

Sustain your growth

Enterprise support

Evaluation of multiple products at small-scale
Further investigations with selected product
Assay validation support
Joint development sessions to improve processes between our companies
High supply security of the material you need in your regular manufacturing
Manufacturing scale up support
Joint business reviews and logistics planning

Product support

Samples of different bead types
Samples coupled with antibody/antigen for rapid screening
Customized protocols
Borderline material
Flexible volumes
Customized products
Bead material from multiple batches for assay validation
Supply security through long-term commercial supply agreement (CSA)
Full Quality Assurance Agreement (QAA)
High supply security
Very low batch to batch variation

Technical support

Technical support
Custom projects
Seminars
Assay automation support
Instrument supplier partnerships

Dynabeads products that support immunodiagnostic assay development

Product	Characteristics	Selected applications
Dynabeads M-270 Epoxy	Hydrophilic bead surface coating with low non-specific protein binding Freeze dried monosized beads; easy to disperse in buffer before use Size: 2.8 micrometer Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required Low aggregation	Immunoassays and immunodiagnostics Protein and peptide purification Immunoprecipitation
Dynabeads MyOne Epoxy	Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding Freeze dried monosized beads, easy to disperse in buffer before use Size: 1 micrometer Low sedimentation rate and higher reaction kinetics compared to M-270 beads Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required	Immunoassays and immunodiagnostics Protein and peptide purification Immunoprecipitation
Dynabeads MyOne Tosylactivated	Hydrophobic bead surface Size: 1 micrometer, monodisperse Iron content (ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Active chemical functionality: 0.04-0.06 mmol/g beads Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads M-280 Tosylactivated	Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups Size: 2.8 micrometer, monodisperse Iron content (ferrites): 12% (17%) Active chemical functionality: 0.1-0.2 mmol/g beads Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads MyOne Streptavidin T1	Hydrophobic bead surface Based on tosylactivated beads (T1) Size: 1 micrometer, monodisperse BSA as blocking protein Isoelectric point: pH 5.0 Low charge (-10 mV, at pH 7) Iron content (ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Free biotin binding: > 1,100-1,700 pmoles/mg beads Binding biotinylated Ig: 40-50 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads M-280 Streptavidin	Hydrophobic bead surface Based on tosylactivated beads Size: 2.8 micrometer, monodisperse BSA as blocking protein Isoelectric point: pH 5.0 Low charge (-10 mV, at pH 7) Iron content (ferrites): 12% (17%) Free biotin binding: 650-900 pmoles/mg beads Binding biotinylated Ig: 5-10 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Protein and peptide purification Phage display and biopanning Cell isolation
Dynabeads M-280 Sheep anti-Rabbit IgG	Hydrophobic bead surface Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses Size: 2.8 micrometer, monodisperse Iron content (ferrites): 12% (17%) Mostly Fc reactive Binding capacity: 0.1-1 µg Ig/10⁷ beads	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell isolation
Dynabeads M-270 Streptavidin	Hydrophilic bead surface Based on carboxylic acid beads Size: 2.8 micrometer, monodisperse No blocking proteins used Isoelectric point: pH 4.5 Highly charged (-50 mV, at pH 7) Iron content (ferrites): 14% (20%) Low aggregation of beads in high salt solutions Free biotin binding: 650-1,350 pmoles/mg beads Binding biotinylated Ig: 5-10 µg/mg beads	Immunoassays and immunodiagnostics with hydrophobic targets Isolation of biotinylated molecules Sequence specific DNA/RNA capture in nucleic acid based diagnostics For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
Dynabeads M-270 Carboxylic Acid	Hydrophilic bead surface with carboxylic acid groups Size: 2.8 micrometer, monodisperse Iron content (ferrites): 14% (20%) Very low aggregation Active chemical functionality 150 µmol/g beads	Immunoassays and immunodiagnostics Sequence specific DNA/RNA capture in nucleic acid based diagnostics Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
Dynabeads MyOne Streptavidin C1	Hydrophilic bead surface Based on carboxylic acid beads (C1) Size: 1 micrometer, monodisperse No blocking proteins used Tween 20 in the buffer Isoelectric point: pH 5.2 Medium charged (-35 mV, at pH 7) Iron content (ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Very low aggregation Free biotin binding: > 2,500 pmoles/mg beads Binding biotinylated Ig: 20-25 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Sample preparation of proteins for mass spectrometry Sequence specific DNA/RNA capture in nucleic acid based diagnostics

Benefits of partnership

There’s a reason Thermo Fisher Scientific already works with some of the world’s leading diagnostic companies.

Premium products: All Dynabeads products are validated with a unique level of reproducibility within and between batches. Patented manufacturing processes ensure careful control of bead parameters and product properties, securing their performance.

Unrivaled bead manufacturing: World-class manufacturing facilities can manufacture batches ranging in size from 1 g up to 2 kg with close to zero off-grade batches and flexible production scales. Meets relevant regulatory and quality systems compliance.

Comprehensive technical support: Extensive proprietary knowledge enables customized product development, decreases quality control costs, and increases manufacturing efficiency. Hands-on support from R&D scientists.

Additional information