How have Dynabeads Plus helped immunoassay development?

Dynabeads Plus team enhanced the sample preparation process for an Alzheimer’s disease diagnostic.


Dynabeads immunodiagnostic product benefits include

  • High capacity & wide dynamic range
  • High signal-to-noise ratio
  • Adaptable to any known detection method & assay methodology
  • Efficient & reproducible ligand immobilization
  • Easy assay development
  • Direct or indirect capture approach
  • No magnetic remanence
  • Excellent kinetics & shorter assay times
  • Reproducible automated behavior
  • Fast and efficient washing
  • Increased sensitivity
  • Long reagent stability on the immunoassay analyzer
  • Easy handling in manufacturing and shortened assay development time

Assay Lifecyle Support

Business support

  • Evaluation of multiple products at small-scale

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols

Enterprise support

  • Evaluation of multiple products at small-scale
  • Collaboration and innovation with high quality products

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Bead material for robustness testing
  • Borderline bead material
  • Flexible supply volumes
  • Customized products
  • Short-term licensing and commercial supply quote agreements
  • Basic Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies
  • High supply security of the material you need in your regular manufacturing
  • Manufacturing scale up support
  • Joint business reviews and logistics planning

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)
  • High supply security
  • Very low batch to batch variation

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support 
  • Instrument supplier partnerships

Business support

  • Evaluation of multiple products at small-scale

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols

Enterprise support

  • Evaluation of multiple products at small-scale
  • Collaboration and innovation with high quality products

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Bead material for robustness testing
  • Borderline bead material
  • Flexible supply volumes
  • Customized products
  • Short-term licensing and commercial supply quote agreements
  • Basic Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies
  • High supply security of the material you need in your regular manufacturing
  • Manufacturing scale up support
  • Joint business reviews and logistics planning

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)
  • High supply security
  • Very low batch to batch variation

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support 
  • Instrument supplier partnerships

ProductCharacteristicsSelected applications
Dynabeads
M-270
Epoxy
  • Hydrophilic bead surface coating with low non-specific protein binding
  • Freeze dried monosized beads; easy to disperse in buffer before use
  • Size: 2.8 micrometer
  • Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required
  • Low aggregation
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Immunoprecipitation
Dynabeads
MyOne
Epoxy
  • Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding
  • Freeze dried monosized beads, easy to disperse in buffer before use
  • Size: 1 micrometer
  • Low sedimentation rate and higher reaction kinetics compared to M-270 beads
  • Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Immunoprecipitation
Dynabeads MyOne 
Tosylactivated
  • Hydrophobic bead surface
  • Size: 1 micrometer, monodisperse
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Active chemical functionality: 0.04-0.06 mmol/g beads
  • Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation
Dynabeads 
M-280
Tosylactivated
  • Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (ferrites): 12% (17%)
  • Active chemical functionality: 0.1-0.2 mmol/g beads
  • Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation

 

Dynabeads MyOne 
Streptavidin T1
  • Hydrophobic bead surface
  • Based on tosylactivated beads (T1)
  • Size: 1 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Free biotin binding: > 1,100-1,700 pmoles/mg beads
  • Binding biotinylated Ig: 40-50 µg/mg beads

 

  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation

 

Dynabeads 
M-280
Streptavidin

  • Hydrophobic bead surface
  • Based on tosylactivated beads
  • Size: 2.8 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (ferrites): 12% (17%)
  • Free biotin binding: 650-900 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads
  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell isolation
Dynabeads 
M-280
Sheep
anti-Rabbit IgG
  • Hydrophobic bead surface
  • Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses
  • Size: 2.8 micrometer, monodisperse
  • Iron content (ferrites): 12% (17%)
  • Mostly Fc reactive
  • Binding capacity: 0.1-1 µg Ig/107 beads

 

  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell isolation
Dynabeads 
M-270
Streptavidin
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Size: 2.8 micrometer, monodisperse
  • No blocking proteins used
  • Isoelectric point: pH 4.5
  • Highly charged (-50 mV, at pH 7)
  • Iron content (ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Free biotin binding: 650-1,350 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads

 

  • Immunoassays and immunodiagnostics with hydrophobic targets
  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.

 

Dynabeads 
M-270
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (ferrites): 14% (20%)
  • Very low aggregation
  • Active chemical functionality 150 µmol/g beads
  • Immunoassays and immunodiagnostics
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte

Dynabeads 
MyOne 
Streptavidin C1

 


  • Hydrophilic bead surface
  • Based on carboxylic acid beads (C1)
  • Size: 1 micrometer, monodisperse
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (-35 mV, at pH 7)
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Very low aggregation
  • Free biotin binding: > 2,500 pmoles/mg beads
  • Binding biotinylated Ig: 20-25 µg/mg beads
  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Sample preparation of proteins for mass spectrometry
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics

Benefits of partnership

There’s a reason Thermo Fisher Scientific already works with some of the world’s leading diagnostic companies.

Premium products: All Dynabeads products are validated with a unique level of reproducibility within and between batches. Patented manufacturing processes ensure careful control of bead parameters and product properties, securing their performance.

Unrivaled bead manufacturing: World-class manufacturing facilities can manufacture batches ranging in size from 1 g up to 2 kg with close to zero off-grade batches and flexible production scales. Meets relevant regulatory and quality systems compliance.

Comprehensive technical support: Extensive proprietary knowledge enables customized product development, decreases quality control costs, and increases manufacturing efficiency. Hands-on support from R&D scientists.

Additional information

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