| Product |
Characteristics |
Selected Applications |
Dynabeads
M-270
Epoxy |
- Hydrophilic bead surface coating with low non-specific protein binding
- Freeze dried monosized beads. Easy to disperse in buffer before use
- Size: 2.8 micrometer
- Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules . No further surface activation required
- Low aggregation
|
- Immunoassays and immunodiagnostics
- Protein and peptide purification
- Immunoprecipation
|
Dynabeads
MyOne
Epoxy |
- Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding.
- Freeze dried monosized beads, easy to disperse in buffer before use
- Size: 1 micrometer
- Low sedimentation rate and higher reaction kinetics compared to M-270 beads
- Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules . No further surface activation required
|
- Immunoassays and immunodiagnostics
- Protein and peptide purification
- Immunoprecipation
|
Dynabeads MyOne
Tosylactivated |
- Hydrophobic bead surface
- Size: 1 micrometer, monodisperse
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Active chemical functionality: 0.04-0.06 mmol/g beads
- Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.
|
- Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
|
Dynabeads
M-280
Tosylactivated |
- Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups
- Size: 2.8 micrometer, monodisperse
- Iron content (Ferrites): 12% (17%)
- Active chemical functionality: 0.1-0.2 mmol/g beads
- Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.
|
- Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
|
Dynabeads MyOne
Streptavidin T1 |
- Hydrophobic bead surface
- Based on tosylactivated beads (T1)
- Size: 1 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Free biotin binding: > 1,100-1,700 pmoles/mg beads
- Binding biotinylated Ig: 40-50 µg/mg beads
|
- Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
|
Dynabeads
M-280
Streptavidin
|
- Hydrophobic bead surface
- Based on tosylactivated beads
- Size: 2.8 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (Ferrites): 12% (17%)
- Free biotin binding: 650-900 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
|
- Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Protein and peptide purification
- Phage display and biopanning
- Cell isolation
|
Dynabeads
M-280
Sheep
anti-Rabbit IgG
|
- Hydrophobic bead surface
- Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses.
- Size: 2.8 micrometer, monodisperse
- Iron content (Ferrites): 12% (17%)
- Mostly Fc reactive
- Binding capacity: 0.1-1 µg Ig/107 beads
|
- Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell isolation
|
Dynabeads
M-270
Streptavidin
|
- Hydrophilic bead surface
- Based on carboxylic acid beads
- Size: 2.8 micrometer, monodisperse
- No blocking proteins used
- Isoelectric point: pH 4.5
- Highly charged (-50 mV, at pH 7)
- Iron content (Ferrites): 14% (20%)
- Low aggregation of beads in high salt solutions
- Free biotin binding: 650-1,350 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
|
- Immunoassays and immunodiagnostics with hydrophobic targets
- Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
|
Dynabeads
M-270
Carboxylic Acid |
- Hydrophilic bead surface with carboxylic acid groups
- Size: 2.8 micrometer, monodisperse
- Iron content (Ferrites): 14% (20%)
- Very low aggregation
- Active chemical functionality 150 µmol/g beads
|
- Immunoassays and immunodiagnostics
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
|
Dynabeads
MyOne
Streptavidin C1
|
- Hydrophilic bead surface
- Based on carboxylic acid beads (C1)
- Size: 1 micrometer, monodisperse
- No blocking proteins used
- Tween 20 in the buffer
- Isoelectric point: pH 5.2
- Medium charged (-35 mV, at pH 7)
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Very low aggregation
- Free biotin binding: > 2,500 pmoles/mg beads
- Binding biotinylated Ig: 20-25 µg/mg beads
|
- Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Sample preparation of proteins for mass spectrometry
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
|
