Dynabeads® M-270 Epoxy are monosized 2.8-µm superparamagnetic polystyrene beads coated with a cross-linked layer of hydrophilic polyether. Epoxy functional groups allow for covalent coupling of antibodies or other proteins, peptides, lectins, haptens, or functional enzymes onto a non-charged bead surface through amine or thiol groups with no further activation of the surface. No blocking protein is needed during the preparation, and standard assay diluents may be used. The Dynabeads®-ligand complex can be used for immunoassays or any other application requiring an active biomolecule conjugated to a magnetic solid support.
Dynabeads® magnetic beads are widely used as a solid-phase for presenting antibodies or antigens in automated clinical immunoassay systems, where the combination of specific antigens or antibodies and the superparamagnetic properties of the beads provide rapid reaction kinetics both in the coating process, separation, and during washing of the analyte.
The product is provided in a freeze-dried format and meets reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.
• Efficient and reproducible immobilization of antibodies (or other ligands) • Easy and reproducible handling in manufacturing • Rapid separation and washing • Reproducible behavior in automation • Easy coupling without need for protein blocking • Freeze-dried beads • High activity of bound antibody and antigen • Easy assay optimization • Well-suited for binding of small antigens such as synthetic or recombinant peptides • Generally low background and high signal-to-noise ratio
Applications Ideally suited for magnetic bead-based immunoassays requiring a low background signal. Also suited for any application involving immunoaffinity binding to antibody or antigen immobilized on a magnetic surface.
Note: This particular product format is for large volume customers, available on an OEM basis. This product is also available in smaller volumes (14301D and 14302D).
For Research Use Only. Not for use in diagnostic procedures.