| Product |
Characteristics |
Selected Applications |
Dynabeads MyOne
SILANE |
- Monosized beads with an optimized silica-like surface chemistry
- Highly reproducible binding of both DNA and RNA
- High specific surface area
(approx. 20 m2/g)
- Size: 1 micrometer, monodisperse
- Isoelectric point: pH 3.5
- Increased iron content compared to the traditional Dynabeads MyOne product range, and hence a better mobility to magnet
- Low sedimentation rate and favorable reaction kinetics
- Flexible starting volumes
|
- Isolation of total nucleic acids (DNA/RNA)
- From biological samples (serum/plasma, human whole blood etc.)
- Well suited for automated applications
- Validation and customization (bead, buffer, protocol or format) available upon request.
- Adjusted to customer requirements on a custom OEM basis
|
Dynabeads
SILANE
viral NA |
- Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of viral DNA/RNA
- High sensitivity for viral DNA and RNA. E.g. can detect HBV DNA in a 200 µl serum sample containing only 40 infectious units
|
- Isolation of viral DNA/RNA from human serum/plasma samples
- Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
|
Dynabeads
SILANE
genomic DNA |
- Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of genomic DNA
- High integrity and purity of isolated DNA
- Typically isolates 10 µg DNA from a 350 µl blood sample using 50 µl (2 mg) Dynabeads
- Linear range of DNA yield relative to White Blood Cell (WBC) counts
|
- Isolation of genomic DNA from human whole blood
- From fresh/frozen blood samples, and with a wide range of anticoagulants
|
| Dynabeads Oligo(dT)25 |
- Hydrophilic bead surface
- Size: 2.8 micrometer
- Iron content (Ferrites): 14% (20%)
- Amount of coupled oligo-dT25 probe:
70 pmol/mg beads
- Capture of polyA mRNA:> 2 µg/mg beads
|
- Capture of polyA+ mRNA directly from crude starting samples
- Build a solid-phase cDNA library
- Specific capture of nucleic acid targets (using capture probes with oligo-dA tail)
|
Dynabeads
M-270
Streptavidin |
- Hydrophilic bead surface
- Based on carboxylic acid beads
- Size: 2.8 micrometer, monodisperse
- Isoelectric point: pH 4.5
- Highly charged (-50 mV, at pH 7)
- Iron content (Ferrites): 14% (20%)
- Low aggregation of beads in high salt solutions
- Free biotin binding: 650-1,350 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
|
- Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Preparation of single-stranded DNA.
- For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
- Immunodiagnostics with hydrophobic targets
|
Dynabeads
M-280
Streptavidin
|
- Hydrophobic bead surface
- Based on tosylactivated beads
- Size: 2.8 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (Ferrites): 12% (17%)
- Free biotin binding: 650-900 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
|
- Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Purification of DNA/RNA binding proteins
- Protein purification, phage display and biopanning
- Immunodiagnostics
|
Dynabeads MyOne
Streptavidin C1 |
- Hydrophilic bead surface
- Based on carboxylic acid beads (C1)
- Size: 1 micrometer, monodisperse
- No blocking proteins used
- Tween 20 in the buffer
- Isoelectric point: pH 5.2
- Medium charged (-35 mV, at pH 7)
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to
M-280/M-270 beads
- Very low aggregation
- Free biotin binding: > 2,500 pmoles/mg beads
- Binding biotinylated Ig: 20-25 µg/mg beads
|
- Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Preparation of single-stranded DNA
- High throughput nucleic acid clean-up protocols
- Well suited for automated applications.
|
Dynabeads MyOne
Carboxylic Acid |
- Hydrophilic bead surface with carboxylic acid groups
- Size: 1 micrometer, monodisperse
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to
M-280/M-270 beads
- Very low aggregation
- Active chemical functionality: 0.6 mmol/g beads
- Coupling capacity: 20-25 µg proteins or 700 pmol oligonucleotides per mg beads
|
- Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
- Well suited for automated applications
|
Dynabeads
M-270
Carboxylic Acid |
- Hydrophilic bead surface with carboxylic acid groups
- Size: 2.8 micrometer, monodisperse
- Iron content (Ferrites): 26% (37%)
- Very low aggregation
- Active chemical functionality 150 µmol/g beads
|
- Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
- Well suited for automated applications
|
Dynabeads MyOne
Tosylactivated
|
- Hydrophobic bead surface
- Size: 1 micrometer, monodisperse
- Iron content (Ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to
M-280/M-270 beads
- Active chemical functionality: 0.04-0.06 mmol/g beads
|
- Immobilization via primary amino- or sulphydryl groups.
- Protein purification, phage display and biopanning
- Immunodiagnostics
|
Dynabeads
M-280
Tosylactivated
|
- Hydrophobic bead surface with
p-toluene-sulfonyl (tosyl) groups
- Size: 2.8 micrometer, monodisperse
- Iron content (Ferrites): 12% (17%)
- Active chemical functionality: 0.1-0.2 mmol/g beads
|
- Immobilization via primary amino- or sulphydryl groups
- Protein purification, phage display and biopanning
- Immunodiagnostics
|
