How have Dynabeads Plus helped Molecular Diagnostic development?

See an example of working with Dynabeads and molecular diagnostic products to measure the impact of radiation exposure.

  • High capacity and wide dynamic range
  • High signal-to-noise ratio
  • Adaptable to any known detection method and assay methodology
  • No magnetic remanence
  • Reproducible automated behavior without mixing
  • Fast and efficient washing
  • Increased sensitivity
  • Easy handling in manufacturing and shortened assay development time

Assay lifecycle support

Business support

  • Evaluation of multiple products at small-scale

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols

Enterprise support

  • Evaluation of multiple products at small-scale
  • Collaboration and innovation with high quality products

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Bead material for robustness testing
  • Borderline bead material
  • Flexible supply volumes
  • Customized products
  • Short-term licensing and commercial supply quote agreements
  • Basic Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies
  • High supply security of the material you need in your regular manufacturing
  • Manufacturing scale up support
  • Joint business reviews and logistics planning

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)
  • High supply security
  • Very low batch to batch variation

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support 
  • Instrument supplier partnerships

Business support

  • Evaluation of multiple products at small-scale

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols

Enterprise support

  • Evaluation of multiple products at small-scale
  • Collaboration and innovation with high quality products

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Bead material for robustness testing
  • Borderline bead material
  • Flexible supply volumes
  • Customized products
  • Short-term licensing and commercial supply quote agreements
  • Basic Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support
  • Instrument supplier partnerships

Enterprise support

  • Evaluation of multiple products at small-scale
  • Further investigations with selected product
  • Assay validation support
  • Joint development sessions to improve processes between our companies
  • High supply security of the material you need in your regular manufacturing
  • Manufacturing scale up support
  • Joint business reviews and logistics planning

Product support

  • Samples of different bead types
  • Samples coupled with antibody/antigen for rapid screening
  • Customized protocols
  • Borderline material
  • Flexible volumes
  • Customized products
  • Bead material from multiple batches for assay validation
  • Supply security through long-term commercial supply agreement (CSA)
  • Full Quality Assurance Agreement (QAA)
  • High supply security
  • Very low batch to batch variation

Technical support

  • Technical support
  • Custom projects
  • Seminars
  • Assay automation support 
  • Instrument supplier partnerships

ProductCharacteristicsSelected applications
Dynabeads MyOne 
SILANE
  • Monosized beads with an optimized silica-like surface chemistry
  • Highly reproducible binding of both DNA and RNA
  • High specific surface area
    (approx. 20 m2/g)
  • Size: 1 micrometer, monodisperse
  • Isoelectric point: pH 3.5
  • Increased iron content compared to the traditional Dynabeads MyOne product range, and hence a better mobility to magnet
  • Low sedimentation rate and favorable reaction kinetics
  • Flexible starting volumes
  • Isolation of total nucleic acids (DNA/RNA)
  • From biological samples (serum/plasma, human whole blood etc.)
  • Well suited for automated applications
  • Validation and customization (bead, buffer, protocol or format) available upon request.
  • Adjusted to customer requirements on a custom OEM basis
Dynabeads 
SILANE
viral NA
  • Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of viral DNA/RNA
  • High sensitivity for viral DNA and RNA, e.g. can detect HBV DNA in a 200 µl serum sample containing only 40 infectious units
  • Isolation of viral DNA/RNA from human serum/plasma samples
  • Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
Dynabeads 
SILANE
genomic DNA
  • Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of genomic DNA
  • High integrity and purity of isolated DNA
  • Typically isolates 10 µg DNA from a 350 µl blood sample using 50 µl (2 mg) Dynabeads
  • Linear range of DNA yield relative to white blood cell (WBC) counts
  • Isolation of genomic DNA from human whole blood
  • From fresh/frozen blood samples, and with a wide range of anticoagulants
Dynabeads Oligo(dT)25
  • Hydrophilic bead surface
  • Size: 2.8 micrometer
  • Iron content (ferrites): 14% (20%)
  • Amount of coupled oligo-dT25 probe: 70 pmol/mg beads
  • Capture of polyA mRNA: >2 µg/mg beads

 

  • Capture of polyA+ mRNA directly from crude starting samples
  • Build a solid-phase cDNA library
  • Specific capture of nucleic acid targets (using capture probes with oligo-dA tail)

 

Dynabeads 
M-270
Streptavidin
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Size: 2.8 micrometer, monodisperse
  • Isoelectric point: pH 4.5
  • Highly charged (-50 mV, at pH 7)
  • Iron content (ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Free biotin binding: 650–1,350 pmoles/mg beads
  • Binding biotinylated Ig: 5–10 µg/mg beads

 

  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Preparation of single-stranded DNA.
  • For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
  • Immunodiagnostics with hydrophobic targets

 

Dynabeads 
M-280
Streptavidin

  • Hydrophobic bead surface
  • Based on tosylactivated beads
  • Size: 2.8 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (ferrites): 12% (17%)
  • Free biotin binding: 650–900 pmoles/mg beads
  • Binding biotinylated Ig: 5–10 µg/mg beads
  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification, phage display and biopanning
  • Immunodiagnostics
Dynabeads MyOne 
Streptavidin C1
  • Hydrophilic bead surface
  • Based on carboxylic acid beads (C1)
  • Size: 1 micrometer, monodisperse
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (-35 mV, at pH 7)
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Very low aggregation
  • Free biotin binding: >2,500 pmoles/mg beads
  • Binding biotinylated Ig: 20–25 µg/mg beads

 

  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Preparation of single-stranded DNA
  • High throughput nucleic acid clean-up protocols
  • Well suited for automated applications

 

Dynabeads MyOne 
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 1 micrometer, monodisperse
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Very low aggregation
  • Active chemical functionality: 0.6 mmol/g beads
  • Coupling capacity: 20–25 µg proteins or 700 pmol oligonucleotides per mg beads

 

  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
  • Well suited for automated applications

 

Dynabeads 
M-270
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (ferrites): 26% (37%)
  • Very low aggregation
  • Active chemical functionality: 150 µmol/g beads
  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
  • Well suited for automated applications
Dynabeads MyOne 
Tosylactivated

 


  • Hydrophobic bead surface
  • Size: 1 micrometer, monodisperse
  • Iron content (ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Active chemical functionality: 0.04–0.06 mmol/g beads
  • Immobilization via primary amino- or sulfhydryl groups.
  • Protein purification, phage display and biopanning
  • Immunodiagnostics
Dynabeads 
M-280
Tosylactivated
  • Hydrophobic bead surface with
    p-toluene-sulfonyl (tosyl) groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (ferrites): 12% (17%)
  • Active chemical functionality: 0.1–0.2 mmol/g beads
  • Immobilization via primary amino- or sulfhydryl groups
  • Protein purification, phage display and biopanning
  • Immunodiagnostics

Benefits of partnership

There’s a reason Thermo Fisher Scientific already works with some of the world’s leading diagnostic companies.

Premium products: All Dynabeads products are validated with a unique level of reproducibility within and between batches. Patented manufacturing processes ensure careful control of bead parameters and product properties, securing their performance.

Unrivaled bead manufacturing: World-class manufacturing facilities can manufacture batches ranging in size from 1 g up to 2 kg with close to zero off-grade batches and flexible production scales. This meets relevant regulatory and quality systems compliance.

Comprehensive technical support: Extensive proprietary knowledge enables customized product development, decreases quality control costs, and increases manufacturing efficiency with hands-on support from R&D scientists.

Resources

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