The Thermo Scientific Volumescope 2 Scanning Electron Microscope (SEM) is our state-of-the-art serial block-face imaging system. Keep control of experiments with easy to use technology and protect a wide range of possible samples with tried and tested solutions for every acquisition step.

Improving large-volume SEM by combining serial block-face imaging with multi-energy deconvolution

Unraveling the complex 3D architecture of cells and tissues in their natural context is crucial for structure-function correlation in biological systems. Serial block-face SEM (SBF-SEM) combines in situ sectioning and imaging of plastic-embedded tissue blocks within the SEM vacuum chamber for automated, 3D reconstruction of large tissue volumes. Axial resolution was once limited by the minimum section thickness, but truly isotropic resolution is now possible with the addition of multi-energy deconvolution.

Following in situ sectioning of the block-face using a diamond knife, the freshly exposed tissue is imaged several times using increasing accelerating voltages. These images are subsequently used in a deconvolution algorithm to derive several optical subsurface layers, forming a 3D subset. By repeating this cycle, the Volumescope 2 SEM offers isotropic datasets with 10 nm Z-resolution.

Scanning Electron Microscopes

3D SEM with the Volumescope 2 SEM

Large volume SEM through combined serial block face imaging and multi-energy deconvolution.


Mouse brain sample imaged on the Volumescope 2 SEM. 
Mouse brain sample imaged on the Volumescope 2 SEM. Fine features such as vesicles and synaptic clefts can be observed. Image courtesy of Dr. Gabor Nyiri, Institute of Experimental Medicine, Budapest, Hungary.
Volume reconstruction of a mouse heart muscle. 
Volume reconstruction of a mouse heart muscle. Data acquired by a combination of physical and virtual slicing in high vacuum mode. Volume = 16 x 15 x 11 μm3 with a nominal resolution of 5 x 5 x 10 nm. Specimen courtesy of Dr. Madesh Muniswamy, Temple University, USA.

Key Features

  • Keep control of experiments with easy to use technology: reuse jobs and system settings and select multiple regions of interest during job acquisition
  • Visualize and navigate during acquisition with Thermo Scientific Amira Live Tracker Software to optimize/control your outcome/Automate large 3D volume acquisitions as well as reconstructions
  • Protect valuable samples with tested solutions at every acquisition step: debris trap and swipe features ensure sample quality; low vacuum detector enables imaging of highly charged samples
  • Easy and fast mounting microtome exchange for normal SEM operation or automated array tomography with the addition of Thermo Scientific Maps Software

Specifications

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Electron optics
  • High-resolution field emission SEM column
    • High-stability Schottky field emission gun
    • Compound final lens: a combined electrostatic, field-free magnetic and immersion magnetic objective lens
  • Dual objective lens combining electromagnetic and electrostatic lenses
Source lifetime

24 months

Automation
  • Auto bakeout
  • Auto start
  • No mechanical alignments
  • Automated heated apertures
  • User guidance and column presets
Stage

Double stage scanning deflection

Electron beam
  • Continuous beam current control and optimized aperture
  • Beam current range: 1 pA to 400 nA
    • In serial block-face imaging SEM: 50 pA – 3.2 nA
  • Landing energy range: 20 eV – 30 keV
  • Accelerating voltage range: 200 V – 30 kV
    • In serial block-face imaging SEM: 500 V – 6 kV
  • Resolution at optimum working distance and routine SEM high-vacuum imaging
    • 0.8 nm at 30 keV STEM
    • 0.7 nm at 15 keV
    • 1.0 nm at 1 keV
Chamber
  • Inside width: 340 mm
  • Analytical working distance: 10 mm
  • Ports: 12
Dectectors
  • Serial block-face imaging detectors
    • T1 segmented lower in-lens detector
    • VS-DBS: LoVac lens-mounted BSED
  • T2 in-lens detector
  • Everhart-Thornley SE detector (ETD)
  • IR-CCD
  • Low-vacuum SE detector
  • STEM 3+ – Retractable segmented detector
  • Additional detectors available
Vacuum system
  • Complete oil-free vacuum system
  • 1 × 220 l/s TMP
  • 1 × PVP-scroll
  • 2 × IGP
  • Chamber vacuum (high-vacuum) <6.3 × 10-6 mbar (after 72 hours pumping)
  • Low-vacuum mode up to 50 Pa for charge compensation of non-conductive samples
  • Evacuation time: ≤3.5 minutes
Sample holders
  • Standard multi-purpose holder; uniquely mounts directly onto the stage; hosts up to 18 standard stubs (Ø12 mm), three pre-tilted stubs, two vertical and two pre-tilted row-bar holders
  • Each optional row-bar accommodates six S/TEM grids
  • Wafer and custom holders

See datasheet for full list of specifications including software, accessories and a summary of installation requirements.

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Applications

Pathology_Pancreas_274x180

Pathology Research

Transmission electron microscopy (TEM) is used when the nature of the disease cannot be established via alternative methods. With nano-biological imaging, TEM provides accurate and reliable insight for certain pathologies.

Plant_Biology_Research_Application_274x180_144_DPI

Plant Biology Research

Fundamental plant biology research is enabled by cryo electron microscopy, which provides information on proteins (with single particle analysis), to their cellular context (with tomography), all the way up to the overall structure of the plant (large volume analysis).

Large Volume Analysis

A novel serial block-face imaging (SBFI) solution that combines multi-energy deconvolution scanning electron microscopy (MED-SEM) with in situ sectioning. Automation and ease-of-use functions provide isotropic resolution for large volume samples.

Learn more ›

Large Volume Analysis

A novel serial block-face imaging (SBFI) solution that combines multi-energy deconvolution scanning electron microscopy (MED-SEM) with in situ sectioning. Automation and ease-of-use functions provide isotropic resolution for large volume samples.

Learn more ›


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