Single particle cryo-EM uses the particles extracted from thousands of images to reconstruct 3D structures of macromolecules. The yield of an acquisition session at the microscope depends on the time to set up the run and the speed of the acquisition itself. Thermo Fisher Scientific EPU 2 software is designed to maximize SPA throughput. Its simplified user interface and advanced automation ensure that setting up a data acquisition session is faster and more accessible, allowing users to focus on their research and make efficient use of the microscope.

EPU 2 tightly integrates with EPU Data Management and the optional EPU Quality Monitor. The Thermo Fisher Scientific Athena software platform and the server hardware used by the Falcon 4 and the Selectris Imaging Filter form the basis for this data management solution. It allows users to organize, view, and share single particle analysis projects easily. EPU Quality Monitor analyzes and visualizes EPU 2 data on-the-fly, enabling users to judge image quality of running or completed acquisitions remotely. The combination of EPU 2, EPU Quality Monitor and Data Management helps to generate high-quality results quickly and with confidence.

Find out more by downloading the EPU 2 datasheet below.

Hemoglobin acquired using the Selectris X Imaging Filter
EPU 2 enables the unattended acquisition of thousands of cryo-EM micrographs that are used to reconstruct protein representations, such as the hemoglobin acquired using the Selectris X Imaging Filter, shown here.
Selectris and Selectris X Imaging Filters

Designed for stability and speed, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Thermo Scientific Falcon 4 Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-electron microscopy (cryo-EM) research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software. The Selectris and Selectris X Imaging Filters are available on Thermo Scientific Krios and Glacios Cryo-TEMs.

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Key Features

• Microscope-embedded SPA software solution for automated grid screening and data acquisition, provides a unified user experience across all compatible instruments.
• Improved ease of use and throughput via systematic user guidance, automated alignments, automation of tasks and support of the latest hardware developments.
• EPU 2 tightly integrates with EPU Quality Monitor and Data Management (powered by Athena), enabling remote data access and on-the-fly analysis of data quality.
• EPU 2 is professionally maintained, updated regularly, and supported by our service organization and application specialists.

Software for Electron Microscopes

EPU 2 Software for Single Particle Analysis

Single particle analysis software for cryo-TEM sample screening and data acquisition.


New in EPU 2.8: Automatic preparation of grid squares.

New in EPU 2.5: Load preferences.

New in EPU 2.8: Automatic preparation of grid squares.

New in EPU 2.5: Load preferences.

Applications

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Structural Biology Research
 

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

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Infectious Disease Research
 

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

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Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

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Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

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Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Documents

Datasheets

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Related Products

Krios G4 Cryo-TEM
Glacios Cryo-TEM
Talos Arctica Cryo-TEM
Talos L120C TEM
Selectris
Falcon 4
Vitrobot
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