Biological research is increasingly focusing on unraveling interactive processes and pathways at the molecular level. To obtain the most biologically relevant information, samples must be observed in 3D and in their native, hydrated states. For this, high-resolution cryo transmission electron microscopy (cryo-TEM) is indispensable, as it provides snapshots of molecular structures frozen within a vitrified aqueous environment.

Cryo-electron microscopy single particle analysis (cryo-EM SPA) in particular has become an established technique for 3D image creation based on cryo-TEM data. This is done by averaging large amounts of well-aligned 2D images into a 3D reconstruction.

Thermo Scientific EPU 2 Software is an automated screening and data acquisition tool that has been developed to efficiently collect these large, high-quality datasets. It provides a holistic solution for both identifying suitable areas for data collection and acquiring the necessary number of high-resolution images for 3D reconstruction and processing.

Key Features

• Microscope-embedded software solution for SPA automated grid screening and data acquisition, providing a unified user experience across all compatible instruments.
• Enhanced ease-of-use. Systematic user guidance combined with automated alignments allows for easy operation from one single interface.
• Efficient screening capabilities. Unattended batch screening of up to 12 grids, creating overview atlases and categorizing the ice film quality for guided selection of grid squares.
• Professional software. The software is professionally maintained by a service organization and supported by application specialists.

Software for Electron Microscopes

EPU 2 Software for Single Particle Analysis

Single particle analysis software for sample screening and data acquisition from cryo TEM data.


Bringing powerful automation and reliability to single particle analysis
The square selection pane displays the same atlas overview image, but with all detected grid squares selected by default green.
EPU: Automated Data Acquisition Software for Single Particle Analysis Workflow
Selection of the holes inside the grid squares.
Automated Data Acquisition Software for Single Particle Workflow.
Targeting the template for recording of the selected holes.

Applications

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Structural Biology Research

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

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Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

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Drug Discovery

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

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Electron microscopy services for
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