Tomography 5 Software – a powerful, automated solution for 3D cryo-tomography

Cryo-electron tomography is a high-resolution technique that enables you to image a cell’s molecular machinery at physiological conditions. The in situ capabilities of cryo-tomography allow it to bridge the gap between single particle analysis (SPA) and structural cell biology, linking molecular insights to cellular behavior. Thermo Scientific Tomography 5 Software brings you the latest advances in cryo-tomography, allowing you to acquire valuable 3D data, even on your most challenging vitrified samples. Its new user-friendly interface enables both experienced and new users to jumpstart their tomography data acquisition.

Cryo-electron-tomography-workflow

Tomo Live Software is an optional add-on for Tomography 5 Software that offers automatic on-the-fly reconstruction with data curation for review -- in any browser, anytime, anywhere.

Tomography 5 Software user-friendly interface supports both experienced and new users to jump-start their tomography data acquisition.


Benefits

Reduced learning curve

Reduced learning curve

Reduced learning curve with step-by-step set-up of tomographic data acquisition.

Automated 3D data acquisition

Automated 3D data acquisition

Automated 3D data acquisition for multi-site tomography.

Intuitive features

Intuitive features

Intuitive featureshelp you set up regions for analysis and offer a range of imaging presets, such as feature-of-interest tracking.


Key Features

Multi-site batch tomography

Multi-site batch tomography for automated, 3D data acquisition, ensuring optimal cryo-transmission electron microscope (cryo-TEM) capacity. Navigate to several regions of interest and set up tomograms, which are automatically acquired. The fully automated data acquisition runs as soon as all settings are defined.

Multi-site batch tomography
Dose symmetric tilt scheme

Dose symmetric acquisition scheme

Dose symmetric acquisition scheme ensures optimal electron dose distribution and high resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.

Automatic cassette mapping

Automatic cassette mapping delivers grid quality assessment and lamella identification. Map up to 12 grids stored in the cryo-TEM autoloader system. A low-magnification map of each grid is taken, allowing for grid quality assessment and efficient screening for cryo-lamella regions.

Automatic cassette mapping
Selectris Imaging Filter enabled

Selectris Imaging Filter enabled

The Thermo Scientific Selectris Imaging Filter greatly improves the signal-to-noise ratio (SNR) in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.

Cryo-ET automation with Tomo Live Software

Thermo Scientific Tomo Live Software is an optional add-on for Tomography 5 Software that offers on-the-fly data monitoring through real-time reconstruction of tilt series into 3D volumes. It can automatically perform motion correction of fraction movies, fiducial-free tilt series alignment, and 3D reconstruction during tilt series recording. These reconstructed tomograms allow for impromptu data quality evaluation, identification of high- and low-quality tilt series, and exporting of selected tilt series and reconstructed tomograms for further processing, archiving, and sharing.

Tomo Live Software runs on the microscope’s Data Management Platform server, and the user interface is integrated in the Thermo Scientific Athena™ Software portal.

Cryo-ET data workflow with Tomo Live Software
  • Automatic reconstruction
  • Combine 100% accurate and complete metadata from Thermo Scientific TEMs with smart estimate and parameter refinement for fully automatic 3D reconstruction within 5 minutes of a tilt series recording.

  • Curation
  • Tomo Live Software supports data curation by displaying quality metrics alongside the reconstructed tomograms. Define rulesets (e.g., alignment accuracy) that are applied during data export.

  • Export
  • Curated tomograms can be exported in a number of formats and are ready for segmentation in Thermo Scientific Amira™ Software. The motion-corrected and aligned tilt series are helpful for particle picking and sub-tomogram averaging workflows.

Curation

Service and technical support

Software Deployment

Deployment

We ensure seamless integration of Tomography 5 Software into your own on-premises environment, allowing you to optimize your infrastructure investments.

Techniques

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Related Products

Krios G4 Cryo-TEM
Glacios Cryo-TEM
Talos Arctica Cryo-TEM
Selectris


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