Tomography 5 Software – a powerful, automated solution for 3D cryo-tomography

Cryo-electron tomography is a high-resolution technique that enables you to image a cell’s molecular machinery at physiological conditions. The in situ capabilities of cryo-tomography allow it to bridge the gap between single particle analysis (SPA) and structural cell biology, linking molecular insights to cellular behavior. Thermo Scientific Tomography 5 Software brings you the latest advances in cryo-tomography, allowing you to acquire valuable 3D data, even on your most challenging vitrified samples. Its new user-friendly interface enables both experienced and new users to jumpstart their tomography data acquisition.

Cryo-electron-tomography-workflow

Benefits

Reduced learning curve

Reduced learning curve

Reduced learning curve with step-by-step set-up of tomographic data acquisition.

Automated 3D data acquisition

Automated 3D data acquisition

Automated 3D data acquisition for multi-site tomography.

Intuitive features

Intuitive features

Intuitive features help you set up regions for analysis and offer a range of imaging presets, such as feature-of-interest tracking.


Key Features

Multi-site batch tomography

Multi-site batch tomography for automated, 3D data acquisition, ensuring optimal cryo-transmission electron microscope (cryo-TEM) capacity. Navigate to several regions of interest and set up tomograms, which are automatically acquired. The fully automated data acquisition runs as soon as all settings are defined.

Multi-site batch tomography
Dose symmetric tilt scheme

Dose symmetric acquisition scheme

Dose symmetric acquisition scheme ensures optimal electron dose distribution and high resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.

Automatic cassette mapping

Automatic cassette mapping delivers grid quality assessment and lamella identification. Map up to 12 grids stored in the cryo-TEM autoloader system. A low-magnification map of each grid is taken, allowing for grid quality assessment and efficient screening for cryo-lamella regions.

Automatic cassette mapping
Selectris Imaging Filter enabled

Selectris Imaging Filter enabled

The Thermo Scientific Selectris Imaging Filter greatly improves the signal-to-noise ratio (SNR) in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.


Service and technical support

Software Deployment

Deployment

We ensure seamless integration of Tomography 5 Software into your own on-premises environment, allowing you to optimize your infrastructure investments.

Techniques

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Related Products

Krios G4 Cryo-TEM
Glacios Cryo-TEM
Talos Arctica Cryo-TEM
Selectris


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