Tomography 5 Software – a powerful, automated solution for 3D cryo-tomography

Cryo-electron tomography is a high-resolution technique that enables you to image a cell’s molecular machinery at physiological conditions. The in situ capabilities of cryo-tomography allow it to bridge the gap between single particle analysis (SPA) and structural cell biology, linking molecular insights to cellular behavior. Thermo Scientific Tomography 5 Software brings you the latest advances in cryo-tomography, allowing you to acquire valuable 3D data, even on your most challenging vitrified samples. Its new user-friendly interface enables both experienced and new users to jumpstart their tomography data acquisition.

Cryo-electron-tomography-workflow

Benefits

Reduced learning curve

Reduced learning curve

Reduced learning curve with step-by-step set-up of tomographic data acquisition.

Automated 3D data acquisition

Automated 3D data acquisition

Automated 3D data acquisition for multi-site tomography.

Intuitive features

Intuitive features

Intuitive featureshelp you set up regions for analysis and offer a range of imaging presets, such as feature-of-interest tracking.


Key Features

Multi-site batch tomography

Multi-site batch tomography for automated, 3D data acquisition, ensuring optimal cryo-transmission electron microscope (cryo-TEM) capacity. Navigate to several regions of interest and set up tomograms, which are automatically acquired. The fully automated data acquisition runs as soon as all settings are defined.

Multi-site batch tomography
Dose symmetric tilt scheme

Dose symmetric acquisition scheme

Dose symmetric acquisition scheme ensures optimal electron dose distribution and high resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.

Automatic cassette mapping

Automatic cassette mapping delivers grid quality assessment and lamella identification. Map up to 12 grids stored in the cryo-TEM autoloader system. A low-magnification map of each grid is taken, allowing for grid quality assessment and efficient screening for cryo-lamella regions.

Automatic cassette mapping
Selectris Imaging Filter enabled

Selectris Imaging Filter enabled

The Thermo Scientific Selectris Imaging Filter greatly improves the signal-to-noise ratio (SNR) in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.


Service and technical support

Software Deployment

Deployment

We ensure seamless integration of Tomography 5 Software into your own on-premises environment, allowing you to optimize your infrastructure investments.

Techniques

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Related Products

Krios G4 Cryo-TEM
Glacios Cryo-TEM
Talos Arctica Cryo-TEM
Selectris


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