Tomography 5 Software

Tomo Live Software is an optional add-on for Tomography 5 Software that offers automatic on-the-fly reconstruction with data curation for review -- in any browser, anytime, anywhere.

Tomography 5 Software user-friendly interface supports both experienced and new users to jump-start their tomography data acquisition.

Reduced learning curve

Automated 3D data acquisition

Intuitive features

Multi-site batch tomography

Multi-site batch tomography for automated, 3D data acquisition, ensuring optimal cryo-transmission electron microscope (cryo-TEM) capacity. Navigate to several regions of interest and set up tomograms, which are automatically acquired. The fully automated data acquisition runs as soon as all settings are defined.

Dose symmetric acquisition scheme

Dose symmetric acquisition scheme ensures optimal electron dose distribution and high resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.

Automatic cassette mapping

Automatic cassette mapping delivers grid quality assessment and lamella identification. Map up to 12 grids stored in the cryo-TEM autoloader system. A low-magnification map of each grid is taken, allowing for grid quality assessment and efficient screening for cryo-lamella regions.

Selectris Imaging Filter enabled

The Thermo Scientific Selectris Imaging Filter greatly improves the signal-to-noise ratio (SNR) in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.

• Automatic reconstruction

Combine 100% accurate and complete metadata from Thermo Scientific TEMs with smart estimate and parameter refinement for fully automatic 3D reconstruction within 5 minutes of a tilt series recording.

• Curation

Tomo Live Software supports data curation by displaying quality metrics alongside the reconstructed tomograms. Define rulesets (e.g., alignment accuracy) that are applied during data export.

• Export

Curated tomograms can be exported in a number of formats and are ready for segmentation in Thermo Scientific Amira™ Software. The motion-corrected and aligned tilt series are helpful for particle picking and sub-tomogram averaging workflows.