The Thermo Scientific Krios Rx Cryo-TEM is the flagship of the iSPA Workflow, the first pharma-dedicated solution for single particle analysis (SPA) and structure-based drug design. This high-end microscope is optimized for the drug discovery process with a guaranteed throughput of over 400 high-quality images per hour. The Krios Rx Cryo-TEM is configured for SPA only with a fixed voltage (300 kV), and comes standard equipped with a Falcon 4 Direct Electron Detector, EPU Quality Monitor, and EPU Data Management (powered by Athena Software). The simplified workflow with next-level EPU automations, including unattended multigrid data collection, makes it ideal for use by non-expert microscopists. The pharma-dedicated service package ensures the industry leading productivity and reliability with a 90% system uptime guarantee (with a Falcon 4 detector).

Key Features

Industry leading productivity

Guaranteed throughput of over 400 high-quality images per hour with a Falcon 4 detector—ideal to help you generate new and repeat structures quickly to match your timeline for lead discovery and optimization.

Enhanced ease of use through automation

Setup time reduced to under 30 minutes. Run the microscope unattended—including overnight or over the weekend—to collect near-atomic resolution images from multiple samples of up to 12 grids.

Guaranteed reliability

Accelerate Rx and Advance Rx service packages offer comprehensive support tailored for the pharma industry, including throughput validation and remote performance monitoring, to deliver a 90% instrument uptime guarantee (with a Falcon 4 detector).

Future-proof platform

The automated and programmable loading door enables compatibility with robotic sample loading in the future.

Compact design

With a height less than 3 meters and the standard included iVIS active vibration dampening system, the microscope fits in a standard room, allowing easy installation and avoiding costly room renovations.


Specifications

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Source X-FEG (high-brightness field emission gun)
Accelerating voltage Fixed at 300 kV
Cryo-Autoloader Automated and contamination-free loading of cassettes (up to 12 Autogrids)
Temperature management software
  • Includes liquid nitrogen autofill and cool down after cryo cycle scheduling
  • Automatic Condenser C2 and Objective, factory aligned C1, C3 and SA apertures
Lenses
  • Symmetric Constant-Power™ C-TWIN objective lens
  • Three-condenser lens system for parallel illumination over a wide and variable field-of-view
Stages Cryo-stage with single axis holder for optimized stability and drift performance
Imaging Optics electronics that enable rotation free imaging, and allow for fast switching of modes, magnifications, conditions and techniques, all with minimized stabilization time.
Advanced performance monitoring Self-assessment of microscope status, combined with automated alignments, ensuring optimal experimental conditions
Detector Falcon 4 Direct Electron Detector and Electron Event Representation (EER) data format for lossless data compression, while retaining super-resolution information
AFIS (aberration-free image shift) Shorter relaxation time with shift between grid holes
FFI (fringe-free imaging) Multiple image acquisitions per grid hole
Thermo Scientific EPU 2 Software Automated SPA screening and data acquisition
EPU Quality Monitor On-the-fly measurement of key image quality parameters

EPU Data Management

Organizing, viewing, and sharing single particle cryo-EM data

Structural components
  • Three 24” monitors
  • Hand panels to be placed within 15 meters from the column, or extend up to 300 meters from the column (optional)
Room Size Requirements (L × W × H) 17’ × 22’ × 10’
iVIS (integrated vibration isolation system) Actively dampens vibration frequencies
Energy filter

Gatan BioContinuum energy filter and K3 camera (optional)

Service packages
  • Accelerate Rx
  • Advance Rx (optional)
Style Sheet for Media Tabs

Meet the Krios Rx Cryo-TEM, the flagship of the iSPA Workflow solution, optimized for single particle analysis in structure-based drug design.

Learn how the iSPA Workflow solution can power structural insights that meet the pace of your drug discovery process.

Pharma collaborators share how the Krios Rx Cryo-TEM offers enhanced automation features for optimal data collection and ease of use.

Pharma collaborators share how the Falcon 4 Detector enables highest throughput imaging for a robust workflow, with optimized EER data format.

Hear how the Accelerate Rx and Advance Rx pharma-dedicated service packages offer comprehensive support to ensure industry leading productivity and instrument reliability.

Meet the Krios Rx Cryo-TEM, the flagship of the iSPA Workflow solution, optimized for single particle analysis in structure-based drug design.

Learn how the iSPA Workflow solution can power structural insights that meet the pace of your drug discovery process.

Pharma collaborators share how the Krios Rx Cryo-TEM offers enhanced automation features for optimal data collection and ease of use.

Pharma collaborators share how the Falcon 4 Detector enables highest throughput imaging for a robust workflow, with optimized EER data format.

Hear how the Accelerate Rx and Advance Rx pharma-dedicated service packages offer comprehensive support to ensure industry leading productivity and instrument reliability.

Applications

DrugDiscovery_Appl_274x180

Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›


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Electron microscopy services for
the life sciences

To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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