DXR3 Raman Microscope overview

Access Raman data faster than ever with this easy-to-use, point-and-shoot Raman microscope. Advanced imaging capabilities make the Thermo Scientific DXR3 Raman Microscope faster and more reliable than ever. Users can more confidently use their Raman data, thanks to automatic x-axis calibration which improves reliability and stability. With speed and flexibility from new lasers and gratings, the DXR3 Raman Microscope is ideal for applications including pharmaceutical, academic, advanced polymers, microplastics, and many more.

DXR3 Raman Microscope capabilities

The DXR3 Raman Microscope enables new particle analysis capabilities for quickly identifying and analyzing microparticles, providing high-performance confocal Raman microscopy in a robust, integrated design. Auto-alignment and calibration ensure scientifically accurate measurements without tools or manual procedures, while an advanced spectrograph design with no moving parts simplifies use and make the detection system and calibration robust.


The DXR3 Raman Microscope offers real-time preview, automated fluorescence correction, autoexposure, and cosmic ray rejection. A system status indicator shows the user at a glance that the system is optimized and ready to collect data. In addition, a three-path fine beam auto-alignment maintains peak performance and sampling integrity, while laser power regulation ensures consistent sample excitation over the lifetime of the laser.

Other capabilities include:

  • Pre-aligned and lock-in-place components that use automatic recognition and stored alignment, allowing any user to reconfigure an instrument in seconds
  • Lasers and other components that can be interchanged and shared with every instrument in the DXR3 Raman family
  • Optional, automated polarized Raman capabilities that provide structural information that complements chemical information
  • The ability to add new wavelengths without tools or a service engineer visit

Laser safety on the DXR3 Raman Microscope

The DXR3 Raman Microscope is independently certified as class-1 laser safe. (Note: Optional fiber-optic accessory and some other optional accessories are Class IIIb laser devices requiring laser safety precautions and laser safety eyewear.) The laser is physically blocked from the visual viewing path to prevent exposure to the eyes while viewing.

DQ/IQ/OQ/PQ validation using the DXR3 Raman Microscope

A complete package for DQ/IQ/OQ/PQ validation is available including extensive documentation and automated software protocols. In addition, Omnic D/S software offers 21 CFR Part 11 compliance.

DXR3 Raman Microscope performance overview

The DXR3 Raman Microscope offers spatial resolution as fine as 540 nm resolution and confocal depth resolution as fine as 1.7 μm on an ideal sample. The full spectral range of 3500-50 cm-1 can be captured with a single exposure of the CCD to avoid stitching artifacts. An extended spectral range to 6,000 cm-1 is available for 532 nm laser.


The DXR3 supports multiple excitation lasers: 455 nm, 532 nm high brightness, 532 nm high power, 633 nm high brightness, 633 nm high power, 785 nm high brightness, and 785 nm high power. Automatic fluorescence correction available with all excitation lasers.


The microscope also provides research-quality Olympus viewing optics. Moreover, automatic intensity correction generates spectra that are comparable between instruments and different excitation lasers, and multi-dimensional wavelength calibration provides accurate wavelength calibration across the entire spectral range.


The DXR3 Raman Microscope’s proprietary triplet spectrograph provides exceptional peak shape and maintains focus at all wavelengths. Users can take advantage of software-controlled switching between confocal and high-energy collection modes. And a proprietary algorithm for automated cosmic ray rejection and high-quality laser line filters ensures artifact-free spectra.


Other performance features include:

  • Lasers depolarized to avoid confusing sample orientation effects
  • A Laser Power Regulator that provides active monitoring of laser power and ensures reproducible laser excitation power over the lifetime of lasers and even after replacing lasers
  • Optimized gratings for each excitation laser to avoid performance sacrifices of systems that share gratings for multiple excitation lasers
  • Proprietary auto alignment that ensures the system can be maintained in like-new condition
  • Proprietary Smart-backgrounds that automatically remove CCD dark energy profile


Forensics—Trace evidence and illicit drug identification


Pharmaceutical—polymorphs, particulate contaminants, and diffusion studies


Solar—Analysis of silicon crystallinity, and characterization of photovoltaic materials


Polymers—inclusions and gel defects, weathering effects, tie layers in laminates, and crystallinity


Nanotechnology—Characterize graphene, CNTs, DLC coatings, and other nanostructures


Gemology—rapid ID of colored stones, distinguishing between natural and synthetic diamonds, characterizing inclusions and adulterants”

Academic research

The DXR3 Raman Microscope is useful in material science, biological studies, and many applied research fields.

Additional applications for the DXR3 Raman Microscope include: art restoration and conservation to identify and characterize pigments, resins, glazes, and inks; archaeology to characterize horn, shell, bone, and ceramic artifacts; as well as, failure analysis for characterizing particulates and small features on surfaces.

DXR3 Raman Microscope resources