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EVOS LED Light Cubes
Invitrogen EVOS Light Cubes integrate LEDs and high-performance filters in a novel illumination system that delivers precise control, minimal maintenance, and exceptional reliability.
These interchangeable LED cubes provide:
- Shorter light paths for more efficient fluorescent detection
- Longer bulb lifetime (>50,000 hours) for lower maintenance costs
- Adjustable light intensity to minimize photobleaching
- Continuous light intensity for more consistent results
These LED cubes are interchangeable, have plug-and-play capability and can be easily transferred between the Invitrogen Countess II FL, EVOS FL, and Invitrogen EVOS FL Auto 2 Cell Imaging System.
 Countess II FL Automated Cell CounterFast and accurate cell counting with fluorescence |  EVOS M5000 Imaging SystemSemi-automatic imaging system with safety features and increased flexibility | 
|
| Light Cube* | Ex (nm) | Em (nm) | Key Compatible Dyes | Cat. No. |
|---|---|---|---|---|
| DAPI | 357/44 | 447/60 | Alexa Fluor 350, BFP, DAPI, Hoechst, LysoTracker Blue, NucBlue Dead, NucBlue Live | AMEP4950 |
| GFP | 482/25 | 524/24 | AlexaFLuor 488, CellROX Green, CellTracker Green, CyQuant Direct, FITC, GFP, MitoTracker Green, YOYO-1 | AMEP4951 |
| RFP | 542/20 | 593/40 | Alexa Fluor 555, CellMask Orange, CellROX Orange, CellTracker Orange, Cy3, MitoTracker Orange CMTMRos, pHrodo, RFP, Rhod-2, SYTOX Orange | AMEP4952 |
| Invitrogen Texas Red | 585/29 | 628/32 | Alexa Fluor 594, CellTracker Red CMTPX, DyLight 594, Katushka, Live/Dead Fixable Red, mCherry, MitoTracker Red CMXRos, mKate, Texas Red | AMEP4955 |
| Invitrogen Cy5 | 635/18 | 692/40 | Alexa Fluor 647, Alexa Fluor 660, Cy5, DRAQ 5, Nuc Red Live 647, SYTO 60 | AMEP4956 |
| TagBFP | 390/18 | 447/60 | TagBFP | AMEP4668 |
| CFP | 445/45 | 510/42 | ECFP, lucifer yellow | AMEP4653 |
| YFP | 500/24 | 542/27 | EYFP, acridine orange (+ DNA) | AMEP4654 |
| Invitrogen Cy5.5 | 655/40 | 794/160 | Cy5.5 | AMEP4673 |
| Invitrogen Cy7 | 710/40 | 809/81 | Cy7, IRDye® 800CW | AMEP4667 |
| CFP-YFP | 445/45 | 542/27 | CFP/YFP (for FRET applications) | AMEP4669 |
| AO | 470/22 | 488/20 | Acridine orange (+RNA, simultaneous red/green with FL color) | AMEP4670 |
| AOred | 445/45 | 628/32 | Acridine orange + RNA, CTC formazan, Invitrogen Fura Red (high Ca2+) | AMEP4671 |
| Invitrogen Qdot 525–800 | 445/45 | 515/LP | All Invitrogen Qdot nanocrystals | AMEP4666 |
| Qdot 525 | 445/45 | 525/15 | Qdot 525 | AMEP4657 |
| Qdot 545 | 445/45 | 544/24 | Qdot 545 | AMEP4658 |
| Qdot 565 | 445/45 | 567/15 | Qdot 565 | AMEP4659 |
| Qdot 585 | 445/45 | 588/20 | Qdot 585 | AMEP4660 |
| Qdot 605 | 445/45 | 605/15 | Qdot 605 | AMEP4661 |
| Qdot 625 | 445/45 | 625/15 | Qdot 625 | AMEP4662 |
| Qdot 655 | 445/45 | 655/15 | Qdot 655 | AMEP4663 |
| Qdot 705 | 445/45 | 710/40 | Qdot 705 | AMEP4664 |
| Qdot 800 | 445/45 | 785/62 | Qdot 800 | AMEP4665 |
* Click the Light Cube link (Cat. No.) for a complete list of compatible dyes/reagents.
Optimized EVOS LED light cubes
Improved light cubes
EVOS now offers newly updated and optimized light cubes that feature improved opto-mechanical design and spectrally optimized optical components. The new design results in superior illumination uniformity across the field of view and maximizes signal-to-background ratios even with dim samples. The cubes are backward compatible with the legacy EVOS light cubes described above, and can also be used with the Invitrogen Countess II FL Automated Cell Counter for expanded interchangeability.
Improved stitching to form composite images
Optimized EVOS Light Cubes provide best-in-class multi-field of view stitching performance.
Click image to enlarge
Best-in-class multi-FOV stitching. Human cerebellum cryopreserved tissue section labeled with NucBlue Fixed Cell ReadyProbes Reagent (Cat. No. R37606), mouse anti-beta-3 Tubulin Antibody (Cat. No. 32-2600) detected with Alexa Fluor Plus 488 Goat Anti-Mouse Secondary Antibody (Cat. No. A32723), Alexa Fluor Plus 555 Phalloidin (Cat. No. A30106), and rabbit anti-GFAP Antibody detected with Alexa Fluor Plus 647 Goat Anti-Rabbit Secondary Antibody (Cat. No. 32733). Tissue was mounted in ProLong Glass Antifade Mounting Media (Cat. No. P36980) and images acquired using an EVOS M7000 automated microscope (Cat. No. AMF7000) with a 10x Olympus (Cat. No. AMEP4753) air objective and DAPI, GFP, RFP, Cy5 Optimized EVOS Light Cubes The finished image (bottom left) is a stitched composite of 272 individual fields of view (top left). Individual panels (right) show the channels that make up the composite.
Spectral fidelity across channels
The new EVOS light cubes provide best-in-class spectral fidelity across channels, eliminating undesired bleedthrough.
Click image to enlarge
Spectral fidelity of optimized EVOS Light Cubes. HeLa cells were labeled with a single bright Phalloidin conjugate, fluorophore indicated on left, and imaged using DAPI, GFP, RFP, and Cy5 EVOS Optimized Light Cubes. As shown in the individual panels, even with bright fluorophores and abundant labels such Alexa Fluor Plus labeled actin cytoskeleton, each light cube shows signal only from its intended target with no bleedthrough, due to the spectral optimizations of the LED excitation sources and filters used in Optimized EVOS Light Cubes.
EVOS M7000 stitching in real-time. EVOS M7000 image stitching of 16x9 composite image of neuronal and glial markers in a sagittal mouse brain section.
The Power of LED Illumination
All of the EVOS fluorescence cell imaging systems utilize LED light sources. That means you get high-intensity output over a short light path for the most efficient fluorophore excitation.
- Shorter light path provides better detection of fluorescent signals
- Continuous illumination gives consistent results
- >50,000-hour bulb lifetime lowers your laboratory costs
- Adjustable light intensity reduces photobleaching
| Revolutionary light path By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals. |
Continuous light intensity | Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days. |
Stability Comparison
Less expensive to own and maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means significant savings in the overall upkeep of your instrument.
What light sources really cost
EVOS hard-coated filter sets for higher transmission efficiency
Hard-coated filter sets are more expensive, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS system’s hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.
TRANSMISSION EFFICIENCY COMPARISON
Superior transmission efficiencies are observed using hard-coated filters on the EVOS instruments, compared to soft-coated filters. Excitation filter (purple), emission filter (red), dichroic mirror (green); Alexa Fluor 488 excitation (blue), Alexa Fluor 488 emission (pink).
For Research Use Only. Not for use in diagnostic procedures.