EVOS™ Light Cubes integrate LEDs and high-performance filters in a novel illumination system that delivers precise control, minimal maintenance, and exceptional reliability.

These interchangeable LED cubes provide:

  • Shorter light paths for more efficient fluorescent detection
  • Longer bulb lifetime (>50,000 hours) for lower maintenance costs
  • Adjustable light intensity to minimize photobleaching
  • Continuous light intensity for more consistent results

View the EVOS dye compatibility table

Download the EVOS dye compatibility table PDF

These LED cubes are interchangeable, have plug-and-play capability and can be easily transferred between the Countess II FL, EVOS FL, and EVOS FL Auto systems

Invitrogen™ Countess™ II FL Automated Cell Counter

Fast and accurate cell counting with fluorescence


Invitrogen™ EVOS™ FL Cell Imaging System

Simple fluorescence imaging with increased flexibility


Invitrogen™ EVOS™ FL Auto Cell Imaging System

Time-lapse imaging at high resolution

Light Cube* Ex (nm) Em (nm) Key Compatible Dyes   
Cat. No.
DAPI 357/44 447/60 DAPI, Hoechst, BFP  AMEP4650
TagBFP 390/18 447/60 TagBFP AMEP4668
CFP 445/45 510/42 ECFP, lucifer yellow AMEP4653
GFP 470/22 510/42 GFP, Alexa Fluor® 488, FITC, SYBR® Green AMEP4651
YFP 500/24 524/27 EYFP, acridine orange (+ DNA) AMEP4654
RFP 531/40 593/40 RFP, Alexa Fluor® 546, Alexa Fluor® 555, Cy®3, DsRed, Rhodamine Red AMEP4652
Texas Red® 585/29 624/40   Texas Red®, Alexa Fluor® 568, Alexa Fluor® 594, MitoTracker® Red, mCherry, Cy®3.5 AMEP4655
Cy®5 628/40 692/40 Cy®5, Alexa Fluor® 647, Alexa Fluor® 660, DRAQ®5 AMEP4656
Cy®5.5 655/46  794/16  Cy®5.5 AMEP4673
Cy®7 710/40 775/46 Cy®7, IRDye® 800CW AMEP4667
CFP-YFP 445/45 542/27 CFP/YFP (for FRET applications) AMEP4669
AO 442/46 488LP Acridine orange (+RNA, simultaneous red/green with FL color) AMEP4670
AOred 442/46 628/32 Acridine orange + RNA, CTC formazan, Fura Red™ (high Ca2+) AMEP4671
Qdot® 525–800 445/45 525-800 All Qdot® nanocrystals AMEP4666
Qdot® 525 445/45   525/15 Qdot® 525 AMEP4657
Qdot® 545 445/45  545/22 Qdot® 545 AMEP4658
Qdot® 565 445/45   565/16 Qdot® 565 AMEP4659
Qdot® 585  445/45 586/20 Qdot® 585 AMEP4660
Qdot® 605  445/45 605/15 Qdot® 605 AMEP4661
Qdot® 625 445/45   625/15 Qdot® 625 AMEP4662
Qdot® 655 445/45   655/15 Qdot® 655 AMEP4663
Qdot® 705 445/45   705/40 Qdot® 705 AMEP4664
Qdot® 800 445/45   785/62 Qdot® 800 AMEP4665

*Click the Light Cube link for a complete list of compatible dyes/reagents.

The Power of LED Illumination

All of the EVOS fluorescence cell imaging systems utilize LED light sources. That means you get high-intensity output over a short light path for the most efficient fluorophore excitation.

  • Shorter light path provides better detection of fluorescent signals
  • Continuous illumination gives consistent results
  • >50,000-hour bulb lifetime lowers your laboratory costs
  • Adjustable light intensity reduces photobleaching




Revolutionary light path

By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous light intensity


Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days.


Stability Comparison


Less expensive to own and maintain

The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means significant savings in the overall upkeep of your instrument.

What light sources really cost

evos light source compare

EVOS hard-coated filter sets for higher transmission efficiency

Hard-coated filter sets are more expensive, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS system’s hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.


Superior transmission efficiencies are observed using hard-coated filters on the EVOS instruments, compared to soft-coated filters. Excitation filter (purple), emission filter (red), dichroic mirror (green); Alexa Fluor® 488 excitation (blue), Alexa Fluor® 488 emission (pink).