EVOS FLoid Imaging System

Catalog number: 4471136

Designed to be one of the simplest, fastest, and easiest cell imaging systems in the world, the EVOS FLoid captures high-quality, 3-color fluorescent cell images right at your benchtop within a minute. Usage is so intuitive, even novice users can collect data in just a few mouse clicks. The EVOS FLoid is ideal for cell culture applications requiring fluorescence, or for just giving you a quick view of your cells.

Features

The EVOS FLoid system shares common features with other EVOS microscopes while simplifying the choice of options. Instead of a choice of LED light cubes, it comes with fixed blue, green, and red LED light sources and filters compatible with the most common reagents and fluorochromes used in fluorescence microscopy. Instead of a choice of objectives, it comes with a fixed 20x objective that supports 460x optical magnification and up to 1,840x digital zoom.

Easy self-installation

Self-installation in as little as 10 minutes with no maintenance, assembly, alignment, or calibration

Basic color options

3-color fluorescence with focus assist and adjustable focus, brightness, and contrast

Multilingual

Your choice of seven interface languages

Speed

Get images within a minute, without warmup, cool-down, or filter changes

Benchtop

Instrument design blocks ambient light, taking fluorescent cell imaging out of the darkroom and onto your benchtop

Self-contained

No moving parts, long-life LEDs for long-term reliability through day-to-day use

Integrated reagent selection guide

With protocols

Attached printer

Print high-quality color images and paste them right in your lab notebook

Enhanced safety

Many operational functions can be controlled through software, reducing the need to touch the instrument

Videos & demos

Interactive demo

Can it really be that easy? Test drive the EVOS FLoid Cell Imaging Station interface with this interactive virtual demo. Simulate imaging a real cell, overlaying each color, and adjusting brightness and contrast before printing.

View virtual demo

View more EVOS systems videos and demos under Resources.

Featured Images

Induced human pluripotent stem cells

EVOS FLoid. Stains: Lin28A (green), Tubulin (red), Hoechst (blue).

Fixed Indian Muntjac cells

EVOS FLoid. Stains: Phalloidin (green), MitoTracker (red), DAPI (blue).

Autofluorescent fly wing

EVOS FLoid.

Live phagocytosis in MMM cells

EVOS FLoid. Stain: pHrodo E. coli BioParticles (red).

View more EVOS FLoid images in our Image Gallery.

Reagent compatibility

Designed to be one of the simplest, fastest, and easiest cell imaging systems in the world, the EVOS FLoid system captures high-quality, 3-color fluorescent cell images right at your benchtop within a minute. Usage is so intuitive, even novice users can collect data in just a few mouse clicks. The EVOS FLoid system is ideal for cell culture applications requiring fluorescence, or for just giving you a quick view of your cells.

EVOS FLoid system light sources and common compatible dyes

Light source	Excitation (nm)	Emission (nm)	Compatible dyes
Blue	390/40	446/33	DAPI, Hoechst stain, NucBlue, Alexa Fluor 350, BFP
Green	482/18	532/59	GFP, Alexa Fluor 488, Calcein, SYBR Green, FITC, Fluo-4, pHrodo Green
Red	586/15	646/68	RFP, Alexa Fluor 546/555/568, PE, Cy3, MitoTracker Orange, pHrodo Red, Rhodamine Red, DsRed

For a more complete list of compatible reagents and applications, see EVOS FLoid reagent compatibility under Resources.

Specifications

Category	Attribute	Description
Optics	Objective	20X plan fluorite;
	Numerical aperture (N/A)	0.45
	Working distance (WD)	5.9 mm (defined as the tip of the objective to the bottom of a 1.2-mm thick slide)
	Overall magnification	460X (optical)-1840X (with digital zoom)
	Display resolution	1366 x 768 pixels
	Image resolution	1296 x 964 pixels
	Contrast methods	Fluorescence and transmitted light (relief phase)
	Color channels	4 channels (relief phase, blue fluorescence, green fluorescence, red fluorescence)
	Illumination	LED (50,000 hour life), adjustable intensity
	Excitation	Blue channel: 390/40 nm; Green channel: 482/18 nm; Red channel: 586/15 nm
	Emission	Blue channel: 446/33 nm; Green channel: 532/59 nm; Red channel: 646/68 nm
	Stage to condenser	60 nm
	Camera	Sony 1.3MP 1/3” ICX445 EXview HAD CCD
Physical characteristics	Instrument type	Benchtop device for imaging cells
	Instrument dimensions	15.9” (W) x 21.1” (H) x 13.9” (D) 40.4 cm (W) x 53.6 cm (H) x 35.3 cm (D)
	Weight	26 lbs (11.8 kg)
	Operating power	100-240 VAC
	Frequency	50-60 Hz
	Electrical input	5 VDC, 4.15 A
	Operating temperature	4-32ºC
	Operating humidity	<90% (non-condensing)
	Output ports	4 USB
Hardware/Software	Stage	Mechanical XY glide stage that can move within a 5 mm diameter circle
	LCD Monitor	15-inch color; display resolution 1366 x 768 pixels; Image resolution 1296 x 964 pixels; adjustable tilt
	Software	FLoid Cell Imaging Station Software
	Captured images	16-bit monochrome TIFF, PNG, BMP and JPG; 1296 x 964 pixels
	Supplied USB drive	2 Gigabyte

For Research Use Only. Not for use in diagnostic procedures.