LED-based fluorescence as well as brightfield illumination

Catalog number: CX51110

 

The Thermo Scientific CellInsight CX5 is a compact benchtop high-content screening platform, offering automated quantitative cell analysis for every cell biology or screening lab. Designed for researchers looking to increase their screening throughput, the platform offers five multiplexable wavelengths for fluorescence and white-light illumination for colorimetric samples. The system is compatible with 6-well through 1,536 microwell plates integrated with the Orbitor RS2 plate handler for automated plate management and can be used to analyze slides and dishes. For groups requiring high-throughput screening, the CX5 can be automated with robotic handling for hands-free workflows. 

 

With proprietary autofocus and integrated plate-scanning intelligence methods, the CellInsight CX5 HCS Platform brings speed and accuracy to investigate cell populations and phenotypes without sacrificing sensitivity and resolution.

 


Features

In addition to the common features of all high-content screening platforms, the CellInsight CX5 platform offers:

Brightfield capabilities

The CellInsight CX5 instrument utilizes transmitted light, allowing you to explore more cell biology without the restriction of fluorescent dyes.

 

Thermo Fisher Connect™

Thermo Fisher Connect Platform provides scientists with a secure place to store, share and analyze their data.

 

Automation with Cytomat incubators

Thermo Scientific Cytomat incubators have internal database-driven inventory management and device grouping, providing you with a complete solution to the automated, high-throughput analysis.

 

Scalable

The CellInsight CX5 instrument addresses the problem of scale with a continuum of robotic solutions depending on your lab’s needs.


Specifications

Category

Attribute

Description

Optics

Camera

Photometrics High-Resolution Fluorescent Camera:

  • High-resolution, thermoelectrically cooled to 0°C
  • FOV 2208 x 2208-pixel array
  • 4.54-micron pixel size
  • 75% peak Quantum Efficiency (QE)

 

Light source

LED, solid-state five-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):

  • 386/440 (e.g., Alexa Fluor 350 dye)
  • 485/521 (e.g., Alexa Fluor 488 dye)
  • 560/607 (e.g., Alexa Fluor 594 dye)
  • 650/694 (e.g., Alexa Fluor 647 dye)
  • 740/810 (e.g., Alexa Fluor 750 dye)

 

Objectives

Standard (Olympus objectives):

  • 10x (0.30NA)
  • 20x (0.4NA)

Optional (Olympus objectives):

  • 2x (0.08NA)
  • 4x (0.16NA)
  • 40x (0.95NA)
  • 60x (0.90NA)

 

Image compatibility

JPG, BMP, GIF, PNG, TIF, C01, DIB

Physical characteristics

Dimensions

Height: 37.2 cm (14.6 in)

Width: 41.4 cm (16.3 in)

Depth: 59.4 cm (23.4 in)

 

Weight

45 kg (99.2 lb)

System

Data management

Compatible with Thermo Scientific Store Image and Database Management Software

 

Software

Thermo Scientific HCS Studio 4.0 Cell Analysis Software

 

PC

Windows 10 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel™ Xeon™ Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.

Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse.

 

Wattage

300 W


Sample data

8_BPAE-cells-with-the-CX5

BPAE cells imaged using the CellInsight CX5 platform

 

BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).

9_HeLa-cells-with-CX5

HeLa cells imaged using the CellInsight CX5 platform

 

HeLa cells imaged using the CellInsight CX5 platform and stained with Hoechst 33342 (blue) and Alexa Fluor 680 phalloidin (yellow).

CX5-C.-Elegans-BF-+-GFP-10X-600-dpi

Brightfield imaging of C. elegans with CellInsight CX5 instrument

 

Mixed brightfield-widefield imaging of Caenorhabditis elegans in vivo samples using the CellInsight CX5 instrument.

CX5-Multiplexed-Image---20X-600-dpi

Multicolor fluorescent image of HEK293 cells captured using CellInsight CX5 instrument 

 

Widefield immunofluorescent imaging of HEK293 cells using the CellInsight CX5 instrument.


Reagent selection guide

The CellInsight CX5 high-content instrument allows you to take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components allowing you to ask more in-depth biological questions.

Fixed cell labeling

Violet
Blue
Green
Orange/Red
Deep Red
Near IR
Alexa Fluor
350
Alexa Fluor
405
Alexa Fluor
488
Alexa Fluor
555
Alexa Fluor
594
Alexa Fluor
647
Alexa Fluor
750
    Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG   Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
    Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
    Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG   Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
    Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
    Donkey anti-rat IgG   Donkey anti-rat IgG    
    Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG   Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
    Rabbit anti-rat IgG   Rabbit anti-rat IgG    
    Chicken anti-goat IgG   Chicken anti-goat IgG Chicken anti-goat IgG  
    Chicken anti-mouse IgG   Chicken anti-mouse IgG Chicken anti-mouse IgG  
    Chicken anti-rabbit IgG   Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
    Chicken anti-rat IgG   Chicken anti-rat IgG Chicken anti-rat IgG  

Live/fixable cell labeling


For Research Use Only. Not for use in diagnostic procedures.