LED-based fluorescence as well as brightfield illumination

Catalog number: CX51110


The Thermo Scientific CellInsight CX5 is a compact benchtop high-content screening platform, offering automated quantitative cell analysis for every cell biology or screening lab. Designed for researchers looking to increase their screening throughput, the platform offers five multiplexable wavelengths for fluorescence and white-light illumination for colorimetric samples. The system is compatible with 6-well through 1,536 microwell plates integrated with the Orbitor RS2 plate handler for automated plate management and can be used to analyze slides and dishes. For groups requiring high-throughput screening, the CX5 can be automated with robotic handling for hands-free workflows. Browse the featuresspecificationsvideossample data, and reagent selection guide sections.


In addition to the common features of all high-content screening platforms, the CellInsight CX5 platform offers:

Brightfield capabilities

The CellInsight CX5 instrument utilizes transmitted light, allowing you to explore more cell biology without the restriction of fluorescent dyes.


Thermo Fisher Connect™

Thermo Fisher Connect Platform provides scientists with a secure place to store, share and analyze their data.


Automation with Cytomat incubators

Thermo Scientific Cytomat incubators have internal database-driven inventory management and device grouping, providing you with a complete solution to the automated, high-throughput analysis.



The CellInsight CX5 instrument addresses the problem of scale with a continuum of robotic solutions depending on your lab’s needs.







Photometrics High-Resolution Fluorescent Camera:

  • High-resolution, thermoelectrically cooled to 0°C
  • FOV 2208 x 2208-pixel array
  • 4.54-micron pixel size
  • 75% peak Quantum Efficiency (QE)


Light source

LED, solid-state five-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):

  • 386/440 (e.g., Alexa Fluor 350 dye)
  • 485/521 (e.g., Alexa Fluor 488 dye)
  • 560/607 (e.g., Alexa Fluor 594 dye)
  • 650/694 (e.g., Alexa Fluor 647 dye)
  • 740/810 (e.g., Alexa Fluor 750 dye)



Standard (Olympus objectives):

  • 10x (0.30NA)
  • 20x (0.4NA)

Optional (Olympus objectives):

  • 2x (0.08NA)
  • 4x (0.16NA)
  • 40x (0.95NA)
  • 60x (0.90NA)


Image compatibility


Physical characteristics


Height: 37.2 cm (14.6 in)

Width: 41.4 cm (16.3 in)

Depth: 59.4 cm (23.4 in)



45 kg (99.2 lb)


Data management

Compatible with Thermo Scientific Store Image and Database Management Software



Thermo Scientific HCS Studio 4.0 Cell Analysis Software



Windows 10 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel™ Xeon™ Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.

Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse.



300 W

Sample data

BPAE cells imaged using the CellInsight CX5 platform


BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).

HeLa cells imaged using the CellInsight CX5 platform


HeLa cells imaged using the CellInsight CX5 platform and stained with Hoechst 33342 (blue) and Alexa Fluor 680 phalloidin (yellow).

Brightfield imaging of C. elegans with CellInsight CX5 instrument


Mixed brightfield-widefield imaging of Caenorhabditis elegans in vivo samples using the CellInsight CX5 instrument.

Multicolor fluorescent image of HEK293 cells captured using CellInsight CX5 instrument 


Widefield immunofluorescent imaging of HEK293 cells using the CellInsight CX5 instrument.

Reagent selection guide

The Thermo Scientific CellInsight CX7 LED Pro high-content instrument allows you to take advantage of the entire fluorescence spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Learn more about reagents for cell viability, proliferation, and function or reagents to determine cell structure. For more specific applications using assays and high-content platforms, check out Assay Selection.

For Research Use Only. Not for use in diagnostic procedures.