eFluor organic dyes spectra

Antibody conjugates offering superior optical performance

Invitrogen eBioscience eFluor organic dyes were developed specifically for labeling and detection of biomolecules. Each eFluor dye is engineered for superior optical performance in a variety of applications.

Reliable performance in multiple applications

eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy. To make fluorophore selection easier, all eFluor organic dyes are named after their emission wavelength and they are fully compatible with most conventional dyes.

Dual parameter scatter plot
eFluor organic dyes
Dye eFluor 450 eFluor 506
Fluorophore type Organic dye Organic dye
Ex (nm) 401 419
Em (nm) 421 506
Application Flow cytometry Flow cytometry
Conjugates available eFluor 450 primary antibody conjugates

eFluor 450 secondary antibody conjugates

eFluor 506 primary antibody conjugates

Recommended bandpass filter 450/50 510/50
Uses Comparable to Invitrogen Pacific Blue dye Use in place of AmCyan or Horizon V500 dye
Dye eFluor 520 eFluor 570 PE-eFluor 610 PerCP-eFluor 710

Fluorophore type

Organic dye

Organic dye

Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye

Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye

Ex (nm)

475

546

496, 565

488

Em (nm)

535

585

607

710

Application

ICC, IHC

ICC, IHC

FC

FC

Conjugates available

 

eFluor 570 primary antibody conjugates

eFluor 570 secondary antibody conjugates

PE-eFluor 610  primary antibody conjugates

PE-eFluor 610  secondary antibody conjugates

PerCP-eFluor 710 primary antibody conjugates

PerCP-eFluor 710 secondary antibody conjugates

Recommended bandpass filter

535/45

585/40

610/20

695/40

Uses

Alternative to Invitrogen Alexa Fluor 488 dye

Alternative to Invitrogen Alexa Fluor 555 dye

Alternative to Invitrogen PE-Texas Red dye

(Note: PE-eFluor 610 is brighter and requires reduced compensation from far-red channels compared to PE-Texas Red dye)

Alternative to PE-Cyanine™5.5 dye or PerCP-Cyanine™5.5

Dye eFluor 570 eFluor 615
Fluorophore type Organic dye Organic dye
Ex (nm) 546 560
Em (nm) 585 645
Application ICC, IHC ICC, IHC
Conjugates available

eFluor 570 primary antibody conjugates

eFluor 570 secondary antibody conjugates

eFluor 615 primary antibody conjugates

eFluor 615 secondary antibody conjugates

Recommended bandpass filter 585/40 645/75
Uses Alternative to Invitrogen Alexa Fluor 555 dye Alternative to Invitrogen Alexa Fluor 647 dye
Dye eFluor 660 APC-eFluor 780
Fluorophore type Organic dye Organic dye (tandem), resonance energy transfer from APC molecule to eFluor 780 dye
Ex (nm) 633 645
Em (nm) 668 767
Application Flow cytometry, ICC, IHC Flow cytometry
Conjugates available

eFluor 660 primary antibody conjugates

eFluor 660 secondary antibody conjugates

APC-eFluor 780 primary antibody conjugates

APC-eFluor 780 secondary antibody conjugates

Recommended bandpass filter 660/20 780/60
Uses Alternative to APC or Invitrogen Alexa Fluor 647 dye Comparable to APC-Cyanine™7 and Invitrogen APC-Alexa Fluor 750 dye

Resources

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Contact our eBioscience custom services group for antibody conjugation, antibody cocktails, immunoassay development, bulk reagents, and testing services.

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