Precise and extraordinarily flexible
genome editing

TAL effectors are a widely used technology for precise and efficient gene editing in live cells. This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, zebrafish, and mammals. We have recently secured rights to certain proprietary methods related to TALs, clarifying a path for you to move confidently forward and innovate.

Thermo Fisher Scientific offers GeneArt PerfectMatch TALs; TAL effector nucleases with a new design that removes the 5′ base constraint and therefore can be designed to target any desired sequence within the genome. We also offer GeneArt Precision TALs for site-specific delivery of activators, repressors, chromatin modifiers, genomic labels, and cross-linking molecules.

Buy now ›   Learn more about TALEN-based editing ›

Video on TALEN technology

Watch this online tutorial, presented by Dr. Jon Chesnut, Research Fellow and R&D lead, to learn more about GeneArt Precision TALs technology.

Optimal TAL Effector design at the touch of a button

TALEN Design Support Request
 

Need us to create engineered cell lines for you? Let us know.

Table 1. Available effector domains

Product Effector domain Functionalities   Applications

GeneArt PerfectMatch TAL
Fok1 endonuclease Gene targeting
(Native TAL Fok1)
  • Silencing
  • Incorporation of exogenous DNA
Fok1 endonuclease Gene targeting
(Truncated TAL)
  • Silencing
  • Incorporation of exogenous DNA
GeneArt Precision TAL VP16 activator Activation of transcription
(Native TAL VP16)
  • Increasing the expression level of endogenous gene isoforms
VP64 activator Activation of transcription
(Native TAL VP64)
  • Increasing the expression level of endogenous gene isoforms
KRAB repressor Epigenetic repression of transcription
(TAL repressor)
  • Heritable knockdown of gene expression
(MCS) Steric repression & custom design
(Native TAL MCS)
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice
(MCS) Steric repression & custom design
(Truncated TAL MCS)
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice
KRAB = Krüppel associated box; MCS = multiple cloning site; TAL = transcription activator–like (DNA-binding domain).


An essential tool for monitoring the efficiency of your genome editing experiments

When using genome editing tools such as CRISPRs, TALs, or zinc finger nucleases to obtain targeted mutations, it is necessary to determine the efficiency with which these nucleases cleave the target sequence prior to continuing with labor-intensive and expensive experiments. The GeneArt™ Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method to determine the nuclease cleavage efficiency at a given locus.