geneart tals

Precise and extraordinarily flexible
genome editing

TAL effectors are a widely used technology for precise and efficient gene editing in live cells. This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, zebrafish, and mammals. We have secured rights to proprietary methods related to TALs, clarifying a path for you to move confidently forward and innovate.

Thermo Fisher Scientific offers TAL effector nucleases (TALEN) with a new design that removes the 5′ base constraint and therefore can be designed to target any desired sequence within the genome. These FlexCut TALEN Pairs are now available in a convenient transfection-ready mRNA format to accelerate your gene editing experiments.

TALENs custom designed for your gene editing needs

Enter your designs for easy online ordering.

Need us to create engineered cell lines for you? Let us know.

Comparison of HDR for a single SNP in three genes
Figure 1. Comparison of HDR for a single SNP in three genes. Three pairs of TALEN mRNAs were screened for cutting at –4, 0, and +4 bases from the desired edit for each SNP. The best-performing pair was used for editing. All CRISPR guide RNAs (gRNAs) that would result in a cut within 25 bases of the desired edit were screened along with the Cas9 protein. The best-performing gRNAs were used for editing.

An essential tool for monitoring the efficiency of your genome editing experiments

When using genome editing tools such as CRISPRs, TALs, or zinc finger nucleases to obtain targeted mutations, it is necessary to determine the efficiency with which these nucleases cleave the target sequence prior to continuing with labor-intensive and expensive experiments. The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method to determine the nuclease cleavage efficiency at a given locus.