Whether using CRISPR, TALEN, or prime editing technology, gene editing confirmation is critical for harnessing the full power of genome engineering and avoiding a large loss of time and money in performing downstream experimentation whose interpretation may be compromised. Applied Biosystems Sanger sequencing and fragment analysis systems and free SeqScreener Gene Edit Confirmation App enable in-depth, highly accurate genomic confirmation and analysis by users of all skill levels, for genomic alterations of all types (deletion, insertion, single-base changes) using a simple sample-to-answer workflow.
In this webinar, Dr. Stephen Jackson, applications senior manager at Thermo Fisher Scientific, discusses the utility of genomic tools in modeling diseases, plants, and animals, as well as the importance of genotypic confirmation of gene edits.
In this webinar series, scientists relate how they harness gene editing technologies to advance their HIV research and rare inherited disease studies including Duchenne muscular dystrophy and MECP2 duplication syndrome.
Unlike phenotypic confirmation, performing genotypic confirmation allows researcher to directly attribute a specific genetic alteration to an experimental outcome, enabling researchers to better understand and confidently interpret their gene editing results across every phase of their workflow. This facilitates the rapid development of more efficient engineering experiments, supports definitive go/no-go decisions on advancing studies relying on engineered cells and/or animal models, and maximizes the safety and efficacy of gene editing-based candidate therapies.
Trust Sanger sequencing and fragment analysis–based methods for rapid gene editing confirmation to more effectively design and optimize gRNAs, screen primary cell pools and single-cell clones, and verify the sequence of the final engineered cells or animal model. Thermo Fisher Scientific offers a range of sequencing and fragment analysis–based methods, cleavage assays, and instruments to meet your speed, throughput, and budgetary needs.
Efficiency and primary pool screening
Secondary clone screening
Targeted confirmation of cells or animal models
Cleavage assay – fragment analysis
TOPO cloning and Sanger sequencing
Determine the range and frequency of gene edits with the free, intuitive SeqScreener Gene Edit Confirmation App.
Investigate genomic tools including sequencing, fragment analysis, and other genetic analysis methods for determining the efficiency and accuracy of CRISPR/Cas9 gene edits.
Thermo Fisher Scientific offers a complete portfolio of products to design, deliver, and confirm gene edits at the phenotypic and genotypic level so you can confidently engineer your cells—all from one place.
Follow the links to learn more about our product offerings for each workflow step. In many cases, the TrueDesign Genome Editor can be used to design and order reagents for the complete editing experiment.
|Design and build
|Detect and validate
For Research Use Only. Not for use in diagnostic procedures.