Search Thermo Fisher Scientific
Differential Ion Mobility for Proteomics
Improve Selectivity. Every Day
The Thermo Scientific FAIMS Pro interface is a next generation differential ion mobility interface that seamlessly works with Thermo Scientific mass spectrometers to further enhance superior selectivity and enable greater proteome profiling while reducing time consuming sample preparation steps.
Better data quality for every proteomic user, every proteomic laboratory
From the discovery of disease biomarkers to the identification of new therapeutic targets, the FAIMS Pro interface helps increase productivity from every proteomic user, and to achieve better data quality through increased selectivity.
Key benefits of the FAIMS Pro interface
| Protect precious samples | Fast, higher quality results | Low cost and improved ease of operation | Focus on science, not setup |
|---|---|---|---|
| The FAIMS Pro technology is the only interface exclusively designed to improve nano, capillary, and microflow applications, allowing you to preserve precious, size-limited samples while achieving higher data quality. | The unique design of FAIMS Pro technology enhances instrument selectivity and detection limits using gas phase fractionation. This results in reduced matrix interference and higher quality data, faster. | Increased selectivity allows you to improve performance and save time by avoiding or reducing offline sample fractionation preparation steps. No consumable parts means lower long-term cost and maintenance. | Designed for inexperienced users to easily install and use. Leverage menu-driven software to design methods using pre-configured parameter recommendations and quickly achieve results. |
See how the FAIMS Pro interface works
The FAIMS Pro interface is easy to set up and deploy to enhance experimental performance and is perfectly suited for nano to micro flow applications (up to 25µL/min). Better selectivity means increased productivity for every proteomic user.
Expanded view of electrodes and entrance plate assembly.
Click to enlarge
Click to enlarge
Ion path through the FAIMS Pro interface from emitter tip to MS capillary. Ions introduced into the FAIMS electrodes are only transmitted to the mass spectrometer when the compound-specific compensation voltage (CV) is set. All non-optimized species are filtered out.
Click to enlarge
Click to enlarge
Generate better quality data and detect more proteins
Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high quality MS and MS/MS data. The FAIMS Pro interface increases analytical performance through gas phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.
OTIT -140 min gradient
Click to enlarge
Comparison of protein and peptide identifications using conventional setup (no FAIMS) or FAIMS with 3 CVs (-50, -65, and -85 V). The addition of the FAIMS Pro interface enables an improvement of 14% in protein IDs and 19% in peptide IDs when compared to not using FAIMS in DDA mode, with a 90-minute nanoflow gradient on the Orbitrap Fusion Lumos MS.
Click to enlarge
A loading curve of HeLa lysate digest with a 90-minute nanoflow gradient and no FAIMS Pro interface compared to FAIMS (CVs at -50 and -70 V) shows improvement in peptide IDs ranging between 25-73% on an Orbitrap Fusion Lumos MS.