Save on HRAM Orbitrap MS for Quantitation and More

Discover the HRAM Orbitrap advantage

For a limited time, receive a discount on the purchase of a new Thermo Scientific™ Q Exactive™ Focus or Thermo Scientific™ Q Exactive™ mass spectrometer and experience the advantages of High Resolution/Accurate Mass (HRAM) Orbitrap MS with highly selective quantitative and qualitative analysis in a single affordable system

The Q Exactive Focus and Q Exactive mass spectrometers bring the benefits of HRAM data, plus proven system reliability, to laboratories performing routine analyses ranging from targeted quantitation to non-targeted screening. Applications include environmental screening and analysis, food safety testing, clinical research, forensic toxicology, metabolomics, and pharmaceutical.

  • Flexibility and ease of use—Detect and quantify all precursor ions in a sample without the need for individual compound tuning and optimization; powered by HRAM Orbitrap technology
  • Resolve analytes from interferences in highly complex matrices—Orbitrap High Resolution/Accurate Mass redefines the standard for accurate quantitation and identification
  • Wide range of concentration—Up to six orders of magnitude of linear dynamic range
  • Analytical confidence—HRAM MS/MS generates spectra confirming compound identification, all with a digital archive for detection and retrospective analysis of all compounds in a sample

Meet today's quantitative challenges with Thermo Scientific™ mass spectrometry solutions and together, we'll transform routine quantitation.

Offers valid only in the U.S., Canada, and direct sales territories in Europe, Middle East & Africa. They cannot be combined with other offers and expire on December 31, 2017.


Why HRAM Orbitrap?
Why should you use HRAM Orbitrap for quantitation?

Triple quads have become more sensitive and faster at performing quantitation using selective reaction monitoring (SRM). Because everyone has their own specific needs, we offer modern, high-performance triple quads for those whose goal is to have dedicated instruments for targeted quantitation. However, you might be looking to do more. If, in addition to targeted quantitation, you require comprehensive analysis of both the known and unknown (expected and unexpected) in the same platform, then high resolution accurate mass (HRAM) using the Orbitrap mass analyzer is the tool for you.

With HRAM-based quantitation in the form of the Q Exactive, which is a quadrupole-Orbitrap, you fundamentally rely on high mass resolution with high mass accuracy for your quantitative and qualitative capabilities.  There is very minimal, if any, optimization required. There is no need to optimize SRMs for every compound on your target list, as needed with triple quadrupole-based quantitation. The high resolution (140,000 on the Q Exactive MS and 70,000 on the Q Exactive Focus MS) and accurate mass (< 1 ppm internal, < 3 ppm external calibration) allows for accurate quantitation and identification of targeted and non-targeted compounds. Thus, quantitation can be performed using full-scan MS. However, sometimes additional selectivity may be required, perhaps due to very complex matrices. If so, there are several ways of doing that. For example, in the Q Exactive MS, prior to the Orbitrap mass analyzer you can isolate an ion of interest in the mass selective quadrupole and perform full scan MS/MS (known as Parallel Reaction Monitoring or PRM) in the Higher-energy Collision Dissociation (HCD) cell measured in the Orbitrap with high mass accuracy and resolution. The figure below shows parallel reaction monitoring, quantitation by full scan MS/MS, in the Q Exactive mass spectrometer.

PRM with the Q Exactive mass spectrometer
Full scan MS/MS quantitation in the Q Exactive.

Above we see (1) precursor ions isolated in the quadrupole which then (2) pass to the Higher-energy Collision Dissociation (HCD) cell for fragmentation followed by (3) HRAM measurement in the Orbitrap. This provides (4) MS/MS identification of the compound and (5) quantitation based on creating accurate mass extracted ion chromatograms from the target compounds.

The tables below provide further reasons why HRAM Orbitrap should be your choice for quantitative analysis and more.

Quantitation using HRAM Orbitrap MS

Quantitation using Triple Quad MS

Well suited for targeted and untargeted applications Well suited for targeted applications
SIM and PRM targeted scan modes for flexibility in quantitation (MS and MS/MS) SIM and SRM modes for high selectivity quantitation; fast SRM good for UHPLC
Screening with HRAM full scan data of all compounds in the sample – unique retrospective analysis Unique fragment scans (precursor ion, neutral loss) for flexibility in quantitation
Accurate mass confirmation of parent & fragment ions (accurate mass, RT, and isotopic pattern) SRM-triggered MS/MS for parent confirmation (QED); ion ratio confirmation
Sensitivity equivalent to TSQ SRM quantitation Highest sensitivity SRM quantitation
Easy method development using full-scan HRAM Automatic compound optimization for convenient ultimate sensitivity method set up (Quick Quan)
6 Orders of linear dynamic range and 5 orders of intrascan dynamic range 6 Orders of linear dynamic range
Fast polarity switching Fast polarity switching
“Quantitation with excellent sensitivity, screening, confirmation capabilities and retrospective data analysis “Quantitation with ultimate sensitivity and robustness”
Ease of Doing Qual/Quan
   

HRAM Orbitrap

Triple Quad

Quan Targeted quantitation Excellent Excellent
Relative Quan
Molecular phenotyping: metabolome and exposome, etc. Excellent Good/Fair
Qual Biomarker ID, fate of drugs and metabolites, pesticides, vet drugs, etc. Excellent Good/Fair
Qual/Quan Both Quan and Qual combined Excellent Good/Fair

What is an Orbitrap?
Why should you use HRAM Orbitrap for quantitation?

1. Full Scan MS/ddMS2 (data dependent MS2)

  • Post-acquisition—extracted ion chromatograms of parent ions of interest
  • Relies on high resolution for selectivity
  • Useful for less complex background
  • No method development/preparation needed

2. t-SIM (targeted SIM)/ddMS2

  • Post-acquisition—extracted ion chromatograms of parent ions of interest
  • Scheduled target list (Rt, m/z)
  • More powerful in medium complexity matrix
  • Minimum method development (e.g., pre-define parent ions, window width)

3. PRM (Parallel Reaction Monitoring)

  • Scheduled target list (Rt, m/z, collision energy)
  • Post-acquisition—extracted ion chromatograms of parent (fragment transitions acquired)
  • Most sensitive and selective even in highly complex matrices

Learn more  about quantitation using HRAM Orbitrap technology.


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