Whether your experiments involve gene expression, microRNA expression, pathogen detection, genotyping, or translocation analysis, the Applied Biosystems StepOne (48-well) and StepOnePlus (96-well) Real-Time PCR Systems provide precise and quantitative measurements with easy-to-use software. The design wizard walks new users through the setup, which includes quantitation method, detection chemistry, ramp speed, nucleic acid template, reaction mix, and sample dilutions.
Figure 1. StepOne™ and StepOnePlus™ Real-Time PCR Systems.
The StepOne System features a 48-well block and 3-color detection. The StepOnePlus System features a 96-well block with six temperature zones and 4-color detection.
Both instruments offer a long-life blue LED-based optical system that records fluorescence from FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes. The StepOnePlus System also accommodates the NED™/TAMRA™ dyes and features VeriFlex™ technology, which combines six independently controllable peltier blocks for enhanced flexibility and precise temperature control.
Applied Biosystems offers a complete series of reagents, kits, and instruments for your research needs from start to finish. These products include RNA isolation kits, TaqMan® Assays (e.g., see page 37, TaqMan Technology: Expanding the Range of Gene Expression Analysis), and real-time PCR systems.
For Research Use Only. Not for use in diagnostic procedures.