The Dynabeads® SILANE genomic DNA kit is designed for highly predictable and consistent isolation of nucleic acids. The specific characteristics of the beads and buffers in the kit are optimized for isolation of genomic DNA from human blood. As an indication of the excellent performance of this kit, one isolation using 50 μl (2 mg) bead will typically isolate 10 μg DNA from a 350 μl blood sample (A260/280 ≥ 1.8, A
≥ 1.4). The kit contains beads and buffers sufficient for 96 isolations.
The Dynabeads MyOne™ SILANE suspension is the key component of this kit. These uniform, monosized ferrimagnetic beads are 1 μm in diameter, and are composed of highly cross-linked polystyrene with evenly distributed magnetic material. The beads are further coated, enclosing the iron oxide inside the beads and presenting a bead surface with optimized silica-like chemistry. The increased magnetic strength of these beads ensure rapid magnetic mobility and efficient isolation of DNA. The beads also feature a low sedimentation rate and favourable reaction kinetics, making them particularly suited for automated assays.
The Dynabeads MyOne™ SILANE component of this kit is available as a separate product, and can be supplied in bulk quantities on an OEM-basis.
The Dynabeads SILANE genomic DNA kit provides an excellent tool for isolation of genomic DNA from human blood, following a simple separation protocol. A buffer is first added to the sample for lysis, followed by incubation with Dynabeads MyOne™ SILANE. The beads with bound DNA are easily pulled to the side of the test tube by using a magnet (e.g. Dynal MPC™) and unbound material is removed by aspiration. The magnetic separation also facilitates simple washing and elution of the isolated DNA. Dynabeads magnetic separation technology is easily adapted to automated liquid handling platforms. Please contact Invitrogen Dynal for further information.
Additional Material Needed
Related Dynabeads Products
- Magnetic device (See magnet recommendations)
- Mixing/rotation device
- Test tubes and pipettes
- Proteinase K at a concentration of 20 mg/ml (e.g. Sigma Cat.No. P2308, dissolved in 10 mM Tris-HCl, pH 8)
- Isopropanol (≥ 99.5%)
- Ethanol (96-100%)
The Dynabeads MyOne™ SILANE component of this kit is also available as a separate product (Cat. no. 370.02D). This product is manufactured under validated production processes, and can be supplied in bulk quantities on an OEM-basis. The Dynabeads SILANE viral NA kit (Cat. no. 370.11D) contains Dynabeads MyOne™ SILANE and specific buffers optimized for sensitive isolation of viral DNA/RNA from human serum/plasma samples. A comprehensive range of Dynabeads for specific capture of nucleic acids is available, across different beads sizes and surface functionalities. Some Dynabeads are precoated with streptavidin, allowing for capture of biotinylated molecules in a wide variety of protocols. Other Dynabeads have a specific surface chemistry for coupling of nucleic acids (e.g hybridization probes/primers) and/or other ligands. Dynabeads products are sold offthe- shelf, and also supplied in bulk quantities on an OEM-basis. The many unique benefits, superior quality and unsurpassed reproducibility of all Dynabeads are renowned in the IVD market. Based on our extensive knowledge and intellectual property (IP) we are able to work with a wide range of customers and partners to develop and validate optimized Dynabeads, buffers and protocols that meet their specific target requirements (e.g. capture of specific virus), downstream applications and regulatory requirements.