Invitrogen™

PureLink™ Genomic DNA Mini Kit

Catalog number:  K182002

Related applications:

DNA Extraction

| SOLiD® Next-Generation Sequencing

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Description

The PureLink® Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink® Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink® Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink® Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink® Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Purification Target: Genomic DNA
Sample Type: Bacteria, Tissue, Cells, Blood
Isolation Technology: Silica Spin Column
Quantity: 250 preps
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100 µl - 1 ml Blood Samples ( Medium-Scale)
Shipping Condition: Room Temperature
Product Line: PureLink™

Contents & storage

• 50 ml PureLink™ Genomic Lysis/Binding Buffer
• 45 ml PureLink™ Genomic Digestion Buffer
• 50 ml PureLink™ Genomic Wash Buffer 1
• 37.5 ml PureLink™ Genomic Wash Buffer 2
• 50 ml PureLink™ Genomic Elution Buffer
• 5 ml RNase A (20 mg/ml)
• 5 ml Proteinase K (20 mg/ml)
• 5 × 50 each PureLink™ Spin Columns with Collection Tubes
• 5 × 100 PureLink™ Collecion Tubes (2.0 ml)

This kit contains enough reagents for 250 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.