Invitrogen™

PureLink™ Genomic DNA Mini Kit

Catalog number: K182002
Invitrogen™

PureLink™ Genomic DNA Mini Kit

Catalog number: K182002
Catalog Number
K182002
also known as K1820-02
Unit Size
250 preps
Price (USD)
728.00
Your Price:
Availability
***
Quantity
Catalog Number
K182000
also known as K1820-00
Unit Size
10 preps
Price (USD)
80.75
Your Price:
Availability
***
Quantity
Catalog Number
K182001
also known as K1820-01
Unit Size
50 preps
Price (USD)
172.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K182002
also known as K1820-02
250 preps
728.00
Your Price:
***
K182000
also known as K1820-00
10 preps
80.75
Your Price:
***
K182001
also known as K1820-01
50 preps
172.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The PureLink® Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink® Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink® Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink® Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink® Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
No. of Reactions
250 Prep
Quantity
250 preps
Format
Microcentrifuge Spin Column
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
100 µl - 1 ml Blood Samples ( Medium-Scale)
Shipping Condition
Room Temperature
Product Line
PureLink™
Purification Target
Genomic DNA
Sample Type
Bacteria, Tissue, Cells, Blood
Isolation Technology
Silica Spin Column
Scale
Mini

Contents & Storage

• 50 ml PureLink™ Genomic Lysis/Binding Buffer
• 45 ml PureLink™ Genomic Digestion Buffer
• 50 ml PureLink™ Genomic Wash Buffer 1
• 37.5 ml PureLink™ Genomic Wash Buffer 2
• 50 ml PureLink™ Genomic Elution Buffer
• 5 ml RNase A (20 mg/ml)
• 5 ml Proteinase K (20 mg/ml)
• 5 × 50 each PureLink™ Spin Columns with Collection Tubes
• 5 × 100 PureLink™ Collecion Tubes (2.0 ml)

This kit contains enough reagents for 250 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.

Figures

Frequently asked questions (FAQs)

Citations & References