Technical Resources

21765 - Ham's F-12 Nutrient Mix

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 7.5 0.1
L-Alanine 89.0 8.9 0.099999994
L-Arginine hydrochloride 211.0 211.0 1.0
L-Asparagine-H2O 150.0 15.0 0.1
L-Aspartic acid 133.0 13.3 0.1
L-Cysteine hydrochloride-H2O 176.0 35.12 0.19954544
L-Glutamic Acid 147.0 14.7 0.1
L-Glutamine 146.0 146.0 1.0
L-Histidine hydrochloride-H2O 210.0 21.0 0.1
L-Isoleucine 131.0 4.0 0.030534351
L-Leucine 131.0 13.1 0.1
L-Lysine hydrochloride 183.0 36.5 0.19945355
L-Methionine 149.0 4.5 0.030201342
L-Phenylalanine 165.0 5.0 0.030303031
L-Proline 115.0 34.5 0.3
L-Serine 105.0 10.5 0.1
L-Threonine 119.0 11.9 0.099999994
L-Tryptophan 204.0 2.04 0.01
L-Tyrosine 181.0 5.4 0.029834256
L-Valine 117.0 11.7 0.1
Vitamins
Biotin 244.0 0.0073 2.9918034E-5
Choline chloride 140.0 14.0 0.1
D-Calcium pantothenate 477.0 0.5 0.001048218
Folic Acid 441.0 1.3 0.0029478457
Niacinamide 122.0 0.036 2.9508196E-4
Pyridoxine hydrochloride 206.0 0.06 2.9126214E-4
Riboflavin 376.0 0.037 9.8404256E-5
Thiamine hydrochloride 337.0 0.3 8.9020777E-4
Vitamin B12 1355.0 1.4 0.0010332103
i-Inositol 180.0 18.0 0.1
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 44.0 0.2993197
Cupric sulfate (CuSO4-5H2O) 250.0 0.0025 1.0E-5
Ferric sulfate (FeSO4-7H2O) 278.0 0.834 0.003
Magnesium Chloride (MgCl2-6H2O) 203.0 122.0 0.6009852
Potassium Chloride (KCl) 75.0 223.6 2.9813335
Sodium Bicarbonate (NaHCO3) 84.0 1176.0 14.0
Sodium Chloride (NaCl) 58.0 7599.0 131.01724
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 142.0 1.0
Zinc sulfate (ZnSO4-7H2O) 288.0 0.863 0.0029965276
Other Components
D-Glucose (Dextrose) 180.0 1802.0 10.011111
Hypoxanthine 136.0 4.0 0.029411765
Linoleic Acid 280.0 0.084 2.9999999E-4
Lipoic Acid 206.0 0.21 0.0010194174
Phenol Red 376.4 1.2 0.0031880978
Putrescine 2HCl 161.0 0.161 0.001
Sodium Pyruvate 110.0 110.0 1.0
Thymidine 242.0 0.7 0.002892562

Reference:

1. Ham, R. G. (1965) Proc. Nat. Acad. Sci., 53, 288.

Related Products:

11765 - Ham's F-12 Nutrient Mix
Cat No: 11765047 , 11765054 , 11765062 , 11765070