Technical Resources

31415 - Leibovitz's L-15

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	200.0	2.6666667
L-Alanine	89.0	225.0	2.52809
L-Alanyl-L-Glutamine	217.0	446.0	2.0552995
L-Arginine	174.0	500.0	2.8735633
L-Asparagine	132.0	250.0	1.8939394
L-Cysteine	121.0	120.0	0.9917355
L-Histidine	155.0	250.0	1.6129032
L-Isoleucine	131.0	250.0	1.908397
L-Leucine	131.0	125.0	0.9541985
L-Lysine	146.0	75.0	0.51369864
L-Methionine	149.0	75.0	0.5033557
L-Phenylalanine	165.0	125.0	0.75757575
L-Serine	105.0	200.0	1.9047619
L-Threonine	119.0	300.0	2.5210085
L-Tryptophan	204.0	20.0	0.09803922
L-Tyrosine	181.0	300.0	1.6574585
L-Valine	117.0	100.0	0.85470086
Vitamins
Choline chloride	140.0	1.0	0.007142857
D-Calcium pantothenate	477.0	1.0	0.002096436
Folic Acid	441.0	1.0	0.0022675737
Niacinamide	122.0	1.0	0.008196721
Pyridoxine hydrochloride	206.0	1.0	0.004854369
Riboflavin 5'-phosphate Na	478.0	0.1	2.0920503E-4
Thiamine monophosphate	442.0	1.0	0.0022624435
i-Inositol	180.0	2.0	0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	140.0	1.2612612
Magnesium Chloride (anhydrous)	95.0	93.7	0.9863158
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	97.67	0.8139166
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Potassium Phosphate monobasic (KH2PO4)	136.0	60.0	0.44117647
Sodium Chloride (NaCl)	58.0	8000.0	137.93103
Sodium Phosphate dibasic (Na2HPO4) anhydrous	142.0	190.0	1.3380282
Other Components
D+ Galactose	180.0	900.0	5.0
Phenol Red	376.4	10.0	0.026567481
Sodium Pyruvate	110.0	550.0	5.0

Reference:

1. Beall, P.T., Karnaky, K.J., Garretson, L.T. et al. (1983) Biochem. Biophys. Acta 763:19 (rat jejumum).

2. Chinchar, G.D. and Sinclair, J.H. (1978) J. Cell. Physiol. 96:333 (amphibian, frog, ICR, 2A).

3. Flamme, I., Albach, K., Muller, S. et al. (1991) Anat. Record, 229:427 (chick embryos).

4. Foxworthy, P.S. and Eacho, P.I. (1986) Toxicol. Ltrs., 30:189 (Rat hepatocytes).

5. Grothe, C., Otto, D. and Unsicker, K. (1989) Neuroscience 31:649 (Rat embryonic E18 septal neurons).

6. Leibovitz, A. (1963) Am. J. Hygiene 78:173.

7. Matsuda, M. (1983) Develop. Biol., 95:219. (Amphibian [newt] neural plates).

8. Middlebrooks, B.L., Mak, P.C. and Ellender, R.D. (1980) Proc. Soc. Exper. Biol. Med., 165:123 (fish fibroblast).

9. Powell, R.L., Dodson, M.V. and Cloud, J.G. (1989) J. Exper. Zool., 50:333 (Fish Satellite cells, mygenic stem cells).

10. Saad, a.H. and El Ridi, R. (1988) Vet. Immunol Imunopath., 18:369 (reptile [lizard] spleen cells).

11. Tate, J.M., Oberpriller, J.O. and Oberpriller, J.C. (1989) Tissue and Cell, 21:335 (Newt cardiac monocytes).

11415 - Leibovitz's L-15
Cat No: 11415049 , 11415056 , 11415064 , 11415114