Leibovitz's L-15 Medium, GlutaMAX™ Supplement

Name: Leibovitz's L-15 Medium, GlutaMAX™ Supplement
SKU: 31415086

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.Read more

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Catalog Number	Quantity
31415086 also known as 31415-086	10 x 500 mL
31415029	500 mL

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Catalog number 31415086

also known as 31415-086

Price (MXN)

Quantity:

10 x 500 mL

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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO₂ equilibration.

This L-15 is modified as follows:

With	Without
• Galactose	• Glucose
• Phenol Red	• HEPES
• GlutaMAX™	• Sodium Bicarbonate
• Sodium Pyruvate

The complete formulation is available.

Gibco™ L-15 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Concentration1 X

Culture EnvironmentNon-CO₂

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality300 - 340 mOsm/kg

Product LineGibco, GlutaMAX

Product TypeLeibovitz's L-15 Medium

Quantity10 x 500 mL

Shelf Life12 Months

ClassificationAnimal Origin-free

Culture TypeMammalian Cell Culture

FormLiquid

SterilitySterile-filtered

With AdditivesGlutaMAX, Phenol Red, Sodium Pyruvate, Galactose

Without AdditivesNo Glucose, No HEPES, No Sodium Bicarbonate

pH7.4 to 7.9

Unit SizeEach

Contents & Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Frequently asked questions (FAQs)

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.