Technical Resources

21180 - Medium 199, 10x

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.0500.06.6666665
L-Alanine89.0250.02.8089888
L-Arginine hydrochloride211.0700.03.3175356
L-Aspartic acid133.0300.02.255639
L-Cysteine hydrochloride-H2O176.01.10.00625
L-Cystine 2HCl313.0260.00.83067095
L-Glutamic Acid-H2O165.0750.04.5454545
L-Glutamine146.01000.06.849315
L-Histidine hydrochloride-H2O210.0218.81.0419048
L-Hydroxyproline131.0100.00.7633588
L-Isoleucine131.0400.03.053435
L-Leucine131.0600.04.5801525
L-Lysine hydrochloride183.0700.03.8251367
L-Methionine149.0150.01.0067114
L-Phenylalanine165.0250.01.5151515
L-Proline115.0400.03.4782608
L-Serine105.0250.02.3809524
L-Threonine119.0300.02.5210085
L-Tryptophan204.0100.00.49019608
L-Tyrosine disodium salt dihydrate261.0580.02.2222223
L-Valine117.0250.02.1367521
Vitamins
Alpha-tocopherol Phosphate702.00.11.4245014E-4
Ascorbic Acid176.00.50.0028409092
Biotin244.00.14.0983607E-4
Choline chloride140.05.00.035714287
D-Calcium pantothenate477.00.12.0964361E-4
Folic Acid441.00.12.2675737E-4
Menadione (Vitamin K3)172.00.15.8139535E-4
Niacinamide122.00.250.0020491802
Nicotinic acid (Niacin)123.00.250.0020325202
Para-Aminobenzoic Acid137.00.50.003649635
Pyridoxal hydrochloride204.00.250.0012254902
Pyridoxine hydrochloride206.00.250.0012135922
Riboflavin376.00.12.6595744E-4
Thiamine hydrochloride337.00.12.967359E-4
Vitamin A (acetate)328.01.00.0030487804
Vitamin D2 (Calciferol)397.01.00.0025188916
i-Inositol180.00.50.0027777778
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.02000.018.018019
Ferric nitrate (Fe(NO3)-9H2O)404.07.20.017821781
Magnesium Sulfate (MgSO4) (anhyd.)120.0970.08.083333
Potassium Chloride (KCl)75.04000.053.333332
Sodium Chloride (NaCl)58.068000.01172.4138
Sodium Phosphate monobasic (NaH2PO4) anhydrous138.01400.010.144928
Other Components
2-deoxy-D-ribose134.05.00.03731343
Adenine sulfate404.0100.00.24752475
Adenosine 5'-phosphate347.02.00.0057636886
Adenosine 5'-triphosphate605.010.00.016528925
Cholesterol387.02.00.005167959
D-Glucose (Dextrose)180.010000.055.555557
Glutathione (reduced)307.00.50.0016286644
Guanine hydrochloride188.03.00.015957447
Hypoxanthine Na158.03.50.022151899
Phenol Red376.4200.00.53134966
Ribose150.05.00.033333335
Sodium Acetate82.0500.06.097561
Thymine126.03.00.023809524
Tween 80®200.0Infinity
Uracil112.03.00.026785715
Xanthine-Na152.03.440.02263158

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.

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