Technical Resources

11595 - Grace's Insect Medium, Unsupplemented

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 650.0 8.666667
L-Alanine 89.0 225.0 2.52809
L-Arginine hydrochloride 211.0 700.0 3.3175356
L-Asparagine 132.0 350.0 2.6515152
L-Aspartic acid 133.0 350.0 2.631579
L-Cystine 240.0 22.0 0.09166667
L-Glutamic Acid 147.0 600.0 4.0816326
L-Glutamine 146.0 600.0 4.109589
L-Histidine 155.0 2500.0 16.129032
L-Isoleucine 131.0 50.0 0.3816794
L-Leucine 131.0 75.0 0.57251906
L-Lysine hydrochloride 183.0 625.0 3.4153006
L-Phenylalanine 165.0 150.0 0.90909094
L-Proline 115.0 350.0 3.0434783
L-Serine 105.0 550.0 5.2380953
L-Threonine 119.0 175.0 1.4705882
L-Tryptophan 204.0 100.0 0.49019608
L-Tyrosine 181.0 50.0 0.2762431
L-Valine 117.0 100.0 0.85470086
beta-Alanine 89.0 200.0 2.247191
Vitamins
Biotin 244.0 0.01 4.0983607E-5
Choline chloride 140.0 0.2 0.0014285714
D-Calcium pantothenate 477.0 0.02 4.192872E-5
Folic Acid 441.0 0.02 4.5351473E-5
Nicotinic acid (Niacin) 123.0 0.02 1.6260162E-4
Para-Aminobenzoic Acid 137.0 0.02 1.459854E-4
Pyridoxine hydrochloride 206.0 0.02 9.708737E-5
Riboflavin 376.0 0.02 5.319149E-5
Thiamine hydrochloride 337.0 0.02 5.934718E-5
i-Inositol 180.0 0.02 1.11111105E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 750.0 6.756757
Magnesium Chloride (MgCl2-6H2O) 203.0 2280.0 11.231527
Magnesium Sulfate (MgSO4-7H2O) 246.0 2780.0 11.300813
Potassium Chloride (KCl) 75.0 4100.0 54.666668
Sodium Bicarbonate (NaHCO3) 84.0 350.0 4.1666665
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 1013.0 7.3405795
Other Components
Alpha-Ketoglutaric acid 146.0 370.0 2.5342467
D-Fructose 180.0 400.0 2.2222223
D-Glucose (Dextrose) 180.0 700.0 3.8888888
Fumaric acid 116.0 55.0 0.47413793
Malic acid 134.0 670.0 5.0
Succinic acid 118.0 60.0 0.5084746
Sucrose 342.0 26680.0 78.011696

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