Grace's Insect Medium, unsupplemented

Catalog number: 11595030

Gibco™  Related applications: Insect Cell Culture

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Description

Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Gibco® Grace's Unsupplemented Insect Medium can be used during transfection of insect cells, or for protein labeling studies (with methionine). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

WithWithout
• L-glutamine • Lactalbumin hydrolysate
• Sodium bicarbonate • Yeastolate
• Methionine

The complete formulation is available. Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate or yeastolate, commonly found in the supplemented version. Gibco® Grace's Unsupplemented Insect Medium is also made without methionine for protein labeling studies.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Unsupplemented Insect Medium requires supplementation with 10% Fetal Bovine Serum (FBS), 50 mg/L methionine, 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Cell Line: Sf21, Sf9
Cell Type: Insect Cells
Glutamine: L-Glutamine
Amino Acids: No Methionine
Supplements: Insect Hemolymph-Free
Product Size: 500 mL
Inorganic Salts: Calcium, Magnesium
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

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