Technical Resources

11667 - Grace's Insect Medium, 2x, Supplemented

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 1300.0 17.333334
L-Alanine 89.0 450.0 5.05618
L-Arginine hydrochloride 211.0 1400.0 6.6350713
L-Asparagine 132.0 700.0 5.3030305
L-Aspartic acid 133.0 700.0 5.263158
L-Cystine 2HCl 240.0 57.36 0.23900001
L-Glutamic Acid 147.0 1200.0 8.163265
L-Glutamine 146.0 1200.0 8.219178
L-Histidine 155.0 5000.0 32.258064
L-Isoleucine 131.0 100.0 0.7633588
L-Leucine 131.0 150.0 1.1450381
L-Lysine hydrochloride 183.0 1250.0 6.830601
L-Methionine 149.0 100.0 0.67114097
L-Phenylalanine 165.0 300.0 1.8181819
L-Proline 115.0 700.0 6.0869565
L-Serine 105.0 1100.0 10.476191
L-Threonine 119.0 350.0 2.9411764
L-Tryptophan 204.0 200.0 0.98039216
L-Tyrosine disodium salt 225.0 100.0 0.44444445
L-Valine 117.0 200.0 1.7094017
beta-Alanine 89.0 400.0 4.494382
Vitamins
Biotin 244.0 0.02 8.196721E-5
Choline chloride 140.0 0.4 0.0028571428
D-Calcium pantothenate 477.0 0.04 8.385744E-5
Folic Acid 441.0 0.04 9.0702946E-5
Nicotinic acid (Niacin) 123.0 0.04 3.2520323E-4
Para-Aminobenzoic Acid 137.0 0.04 2.919708E-4
Pyridoxine hydrochloride 206.0 0.04 1.9417475E-4
Riboflavin 376.0 0.04 1.0638298E-4
Thiamine hydrochloride 337.0 0.04 1.1869436E-4
i-Inositol 180.0 0.04 2.2222221E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 1000.0 9.009009
Magnesium Chloride (anhydrous) 95.0 2138.64 22.512
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 2715.34 22.627834
Potassium Chloride (KCl) 75.0 5600.0 74.666664
Sodium Bicarbonate (NaHCO3) 84.0 700.0 8.333333
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 2026.0 14.681159
Other Components
Alpha-Ketoglutaric acid 146.0 740.0 5.0684934
D-Fructose 180.0 800.0 4.4444447
D-Glucose (Dextrose) 180.0 1400.0 7.7777777
Fumaric acid 116.0 110.0 0.94827586
Lactalbumin hydrolysate 6660.0 Infinity
Malic acid 134.0 1340.0 10.0
Succinic acid 118.0 120.0 1.0169492
Sucrose 342.0 53360.0 156.02339
Yeastolate 6660.0 Infinity

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