Grace's Insect Medium (2X), supplemented This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  11667037

 Related applications:

Insect Cell Culture

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Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). This 2x formulation is intended for use in the agarose overlay when performing the plaque assay. We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

• Lactalbumin hydrolysate
• Yeastolate
• L-glutamine
• Sodium bicarbonate

The complete formulation is available. Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Supplemented Insect Medium, 2x is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate, and is commonly diluted with sterile water, Fetal bovine Serum and agarose before use in the agarose overlay, as described in the product manual.
For Research Use Only. Not for use in diagnostic procedures.


Cell Line: Sf21, Sf9
Cell Type: Insect Cells
Form: Liquid
Glutamine: L-Glutamine
Product Line: Gibco®
Product Size: 100 mL
Supplements: Lactalbumin Hydrolysate, Yeastolate
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture


Manuals & protocols