Technical Resources

11405 - IPL-41 Insect Medium

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 200.0 2.6666667
Hydroxy L-proline 131.0 800.0 6.10687
L-Arginine hydrochloride 211.0 800.0 3.791469
L-Asparagine 132.0 1300.0 9.848485
L-Aspartic acid 133.0 1300.0 9.774436
L-Cystine-2Na 286.0 119.14 0.41657344
L-Glutamic Acid 147.0 1500.0 10.204082
L-Glutamine 146.0 1000.0 6.849315
L-Histidine 155.0 200.0 1.2903225
L-Isoleucine 131.0 750.0 5.7251906
L-Leucine 131.0 250.0 1.908397
L-Lysine hydrochloride 183.0 700.0 3.8251367
L-Methionine 149.0 1000.0 6.7114096
L-Phenylalanine 165.0 1000.0 6.060606
L-Proline 115.0 500.0 4.347826
L-Serine 105.0 200.0 1.9047619
L-Threonine 119.0 200.0 1.6806723
L-Tryptophan 204.0 100.0 0.49019608
L-Tyrosine disodium salt dihydrate 261.0 360.4 1.3808429
L-Valine 117.0 500.0 4.2735043
beta-Alanine 89.0 300.0 3.3707864
Vitamins
Biotin 244.0 0.16 6.557377E-4
Choline chloride 140.0 20.0 0.14285715
D-Calcium pantothenate 477.0 0.008 1.677149E-5
Folic Acid 441.0 0.08 1.8140589E-4
Nicotinic acid (Niacin) 123.0 0.16 0.0013008129
Para-Aminobenzoic Acid 137.0 0.32 0.0023357663
Pyridoxine hydrochloride 206.0 0.4 0.0019417476
Riboflavin 376.0 0.08 2.1276595E-4
Succinic acid 118.0 4.8 0.04067797
Thiamine hydrochloride 337.0 0.08 2.3738873E-4
Vitamin B12 1355.0 0.24 1.7712176E-4
i-Inositol 180.0 0.4 0.0022222223
Inorganic Salts
Ammonium Molybdate ((NH4)6Mo7O24-4H2O) 1236.0 0.04 3.236246E-5
Calcium Chloride (CaCl2) (anhyd.) 111.0 500.0 4.5045047
Cobalt Chloride (CoCl2-6H2O) 238.0 0.05 2.1008404E-4
Cupric chloride (CuCl2-2H2O) 170.0 0.2 0.0011764707
Ferric sulfate (FeSO4-7H2O) 278.0 0.55 0.0019784174
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 918.0 7.65
Manganese chloride (MnCl2-4H2O) 198.0 0.02 1.010101E-4
Potassium Chloride (KCl) 75.0 1200.0 16.0
Sodium Bicarbonate (NaHCO3) 84.0 350.0 4.1666665
Sodium Chloride (NaCl) 58.0 2850.0 49.13793
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 1160.0 8.405797
Zinc chloride (ZnCl2) 136.0 0.04 2.9411764E-4
Other Components
Alpha-Ketoglutaric acid 146.0 29.6 0.20273973
D-Glucose (Dextrose) 180.0 2500.0 13.888889
Fumaric acid 116.0 4.4 0.037931036
Malic acid 134.0 53.6 0.39999998
Maltose 342.0 1000.0 2.9239767
Sucrose 342.0 1650.0 4.8245616

Reference:

1. Schneider, I., (1964) Differentiation of larval Drosophila eye-antennal discs in vitro. J. Exp. Zool., 156:91.

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