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Ki-67 Antibody (MA5-14520) in Flow
Flow cytometry analysis for Ki-67 was carried out using MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Ki-67 Rabbit Monoclonal Antibody (Product ## MA5-14520, red histogram) at a dilution of 1:20 or a matched isotype control (yellow histogram) in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (Product ## A11008) at a dilution of 1:400 for 30 minutes at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents the no-primary-antibody control.
