Kinases screened in CellSensor cells with a ratiometric two-color reporter assay

(A) CellSensor cell lines use ß-lactamase reporter technology to provide a rapid and sensitive method of analyzing activation of signal transduction pathways. (B) Overview of the screening workflow. (C) After stimulation with TNFa, the ratio of green to blue fluorescence decreased in unedited CellSensor NF-?B-bla ME-180 cells. Cells infected with lentiviral particles carrying gRNA that effectively disrupted the NF-?B pathway remained green, with a high ratio of green to blue fluorescence. Complete knockout of TNFR1 was observed in nearly 100% of the cells.

(A) CellSensor cell lines use ß-lactamase reporter technology to provide a rapid and sensitive method of analyzing activation of signal transduction pathways. (B) Overview of the screening workflow. (C) After stimulation with TNFa, the ratio of green to blue fluorescence decreased in unedited CellSensor NF-?B-bla ME-180 cells. Cells infected with lentiviral particles carrying gRNA that effectively disrupted the NF-?B pathway remained green, with a high ratio of green to blue fluorescence. Complete knockout of TNFR1 was observed in nearly 100% of the cells.

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