Bovine pulmonary endothelial cells. MitoTracker® Red CMXRos, SYTO® 16 nucleic acid stain, anti–bovine a-tubulin (mouse monoclonal) and Alexa Fluor® 350 goat anti–mouse IgG.

Bovine pulmonary endothelial cells incubated with the fixable, mitochondrion-selective MitoTracker® Red CMXRos (Cat. no. M7512). The cells were then formaldehyde-fixed, acetone-permeabilized and counterstained using SYTO® 16 green-fluorescent nucleic acid stain (Cat. no. S7578). Microtubules were labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126) and visualized with blue-fluorescent Alexa Fluor® 350 goat anti–mouse IgG (Cat. no. A11045). The multiple-exposure image was acquired using filter sets appropriate for Texas Red® dye, fluorescein and DAPI.

Bovine pulmonary endothelial cells incubated with the fixable, mitochondrion-selective MitoTracker® Red CMXRos (Cat. no. M7512). The cells were then formaldehyde-fixed, acetone-permeabilized and counterstained using SYTO® 16 green-fluorescent nucleic acid stain (Cat. no. S7578). Microtubules were labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126) and visualized with blue-fluorescent Alexa Fluor® 350 goat anti–mouse IgG (Cat. no. A11045). The multiple-exposure image was acquired using filter sets appropriate for Texas Red® dye, fluorescein and DAPI.

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