Transformed mouse fibroblasts (CRE BAG 2 cells).
lacZ mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavidin–alkaline phosphatase conjugate in combination with the ELF 97 alkaline phosphatase substrate, both of which are provided in our ELF® 97 mRNA In Situ Hybridization Kit (Cat. No. E6604, E6605). Cells were counterstained with DAPI (Cat. No. D1306, D3571, D21490) and photographed using a longpass optical filter appropriate for DAPI.
Prostate carcinoma. Go ›
Zebrafish brain. ELF 97 Endogenous Phosphatase Detection Kit. Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Labeled paint probes hybridized to human metaphase chromosomes Go ›