The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.
The yellow-green fluorescence of the ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye. Adult zebrafish cryosections were first stained with Texas Red®-X wheat germ agglutinin (Cat. no. W21405) followed by the ELF® 97 phosphate substrate in the ELF® 97 Endogenous Phosphatase Detection Kit (Cat. no. E6601), and finally the nuclei were counterstained with the blue-fluorescent Hoechst 33342 nucleic acid stain (Cat. no. H1399, H3570, H21492). The triple-exposure image was acquired using bandpass filters appropriate for the Texas Red® dye, ELF® 97 alcohol precipitate and AMCA.
Prostate carcinoma. Go ›
Transformed mouse fibroblasts (CRE BAG 2 cells). Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›