Chick embryo fibroblasts. Actin from rabbit muscle, Alexa Fluor 568 conjugate and coumarin phallacidin.
Chick embryo fibroblasts injected with the Alexa Fluor 568 conjugate of actin from rabbit muscle (A12374). The cells were then fixed and permeabilized, and the filamentous actin was stained with coumarin phallacidin (C606). The double-exposure image was acquired using longpass filter sets appropriate for rhodamine and DAPI. Image contributed by Heiti Paves, Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Estonia.